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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Related Experiment Video

Updated: Dec 29, 2025

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

Evolving data standards for cryo-EM structures.

Catherine L Lawson¹, Helen M Berman, Wah Chiu

¹Institute for Quantitative Biomedicine and Research Collaboratory for Structural Bioinformatics, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854, USA.

Structural Dynamics (Melville, N.Y.)

|February 1, 2020

Summary

This summary is machine-generated.

Electron cryo-microscopy (cryo-EM) structure archiving and validation standards have been developed over two decades by an international collaboration. This work ensures reliable data deposition for biological specimens, mirroring standards in other structural biology fields.

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Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

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Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Published on: January 9, 2015

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Last Updated: Dec 29, 2025

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Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Published on: January 9, 2015

Area of Science:

Structural Biology
Biophysics
Biochemistry

Background:

Electron cryo-microscopy (cryo-EM) is a rapidly advancing technique for determining 3D structures of biological specimens.
The need for robust data archiving and validation standards for cryo-EM data became apparent early in its development.

Purpose of the Study:

To describe the development and coordination of cryo-EM data archiving and validation standards.
To highlight the role of EMDataResource (formerly EMDataBank) and its collaboration with the Protein Data Bank.
To discuss the impact of technological advancements on data standards.

Main Methods:

Establishment of an international scientific collaboration with expertise in cryo-EM and data archiving.
Development of infrastructure for archiving cryo-EM density maps and models.
Promotion of cryo-EM structure validation standards through community engagement.

Main Results:

Over two decades, coordinated efforts have led to established archiving and validation processes for cryo-EM data.
Joint coordination between the Electron Microscopy Data Bank and Protein Data Bank has been crucial.
Evolving technology has necessitated continuous adaptation of data standards.

Conclusions:

The development of cryo-EM data archiving and validation has been a collaborative, community-driven effort.
Established standards ensure the integrity and accessibility of cryo-EM structural data.
Ongoing engagement with the scientific community is vital for advancing cryo-EM data standards.