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Author Spotlight: Advancing Therapeutics with Biocompatible Sodium Alginate Hydrogel Microspheres
Published on: June 7, 2024
Preparation of pH-Responsive Alginate-Chitosan Microspheres for L-Valine Loading and Their Effects on the A40926
Xue Yue1, Bingyu Yan1, Shuai Wang1
1School of Pharmacy, Liaocheng University, No. 1, Hunan Road, Dongchangfu District, Liaocheng, 252000, Shandong, People's Republic of China.
Abstract:
The glycopeptide A40926 biosynthesized by Nonomuraea gerenzanensis is a precursor of the second generation glycopeptide antibiotic dalbavancin. The skeleton of this glycopeptide consists of seven amino acids and is biosynthesized by the NRPS gene module. L-valine, a branched amino acid, is also a significant precursor for A40926 production. This study details the use of pH-responsive alginate-chitosan microspheres loaded with L-valine prepared by internal emulsification gelation. The effects of process and formulation variables on microsphere size, loading capacity, and encapsulation efficiency were investigated. Then, effects on A40926 production by the pH-responsive microspheres were evaluated in a 10-L fermenter. Results demonstrated that use of the pH-responsive microspheres could improve A40926 yield from 465 to 602 mg L-1 in a 10-L scale fermenter.
