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Updated: Dec 29, 2025

Purification of a High Molecular Mass Protein in Streptococcus mutans
Published on: September 14, 2019
Association of Streptococcus mutans collagen binding genes with severe childhood caries
Gurkaran S Lamba1, Delphine Dufour2, S M Hashim Nainar3
1Faculty of Dentistry, Orthodontics, University of Toronto, 124 Edward Street, Toronto, ON, M2G 1G6, Canada.
Objective:
An important factor in the assessment of caries risk is the presence of specific oral microflora, especially Streptococcus mutans. Some S. mutans strains possess proteins capable of binding collagen, such as the Cnm and Cbm proteins. The aim is to determine the presence of S. mutans strains carrying collagen binding proteins in a group of subjects with severe early childhood caries (S-ECC).
Materials And Methods:
S. mutans strains isolated from 15 S-ECC children were analyzed for collagen binding domains (cbd) of the cnm (cbd/cnm) and cbm (cbd/cbm) genes and their ability to bind to collagen.
Results:
S. mutans strains positive for cbd/cnm or cbd/cbm were only found in 3 subjects with the most severe caries profile, with one subject having both cbd/cnm and cbd/cbm, and the other two with one of each. cnm/cbm-positive S. mutans strains bound to collagen substrate more avidly compared with negative S. mutans strains from each of the three groups.
Conclusions:
Our findings of an association between the presence of the collagen binding domains of the cnm/cbm genes in plaque S. mutans and the most aggressive form of caries profile in children offer a potential strategy to identify an individual's risk for caries progression. Our study should be replicated in other settings and communities in longitudinal and longer-term studies.
Clinical Relevance:
Our data offer a potential tool in the caries risk management and assessment in children.
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