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Cell-Based Assays for Modeling Xenogeneic Immune Responses.

Kelly Casós1,2, Roberta Sommaggio1, Magdiel Pérez-Cruz1,3,4

  • 1Infectious Diseases and Transplantation Division, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|February 1, 2020
PubMed
Summary

Investigating xenograft rejection requires complex studies. New cell-based assays using pig cells and human immune cells help understand rejection mechanisms and develop tolerance strategies for xenotransplantation.

Keywords:
CytokinesHumanImmune responseMonocytesNK cellsPigT cells

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Area of Science:

  • Xenotransplantation research
  • Immunology
  • Cellular and molecular biology

Background:

  • Xenotransplantation research faces complexity in molecular, cellular, and in vivo studies.
  • Focus is shifting to cellular immune responses against xenografts after addressing xenoantigens.
  • Understanding pig cell and human leukocyte interactions is crucial for xenograft survival.

Purpose of the Study:

  • To describe cell-based assays for investigating xenograft immune rejection mechanisms.
  • To elucidate cellular and molecular pathways in xenogeneic immune responses.
  • To support the development of strategies promoting xenograft survival.

Main Methods:

  • Development and description of xenogeneic cell-based assays.
  • Utilizing coculture, co-stimulatory, and cytotoxicity assays.
  • Employing pro-inflammatory and anti-inflammatory cytokines to study immune regulation.

Main Results:

  • Established assays facilitate the study of cellular and molecular mechanisms of xenograft rejection.
  • Techniques allow elucidation of key pathways in xenogeneic immune responses.
  • Assays enable investigation of immune response regulation under various conditions.

Conclusions:

  • Described cell-based assays are valuable tools for xenotransplantation research.
  • These methods aid in understanding xenograft rejection and developing tolerance strategies.
  • Assays can monitor in vivo immune responses and support therapeutic development.