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Related Experiment Video

Updated: Dec 29, 2025

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
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Wide area quantitative phase microscopy by spatial phase scanning digital holography.

Myung K Kim

    Optics Letters
    |February 1, 2020
    PubMed
    Summary

    A new spatial phase scanning digital holography technique enables large area quantitative phase microscopy. This method efficiently scans specimens for diverse biological and material science applications.

    Area of Science:

    • Optics and Photonics
    • Microscopy
    • Biophysics

    Background:

    • Quantitative phase microscopy (QPM) is crucial for label-free imaging of transparent specimens.
    • Traditional QPM techniques can be limited in scan area and speed.
    • Digital holographic microscopy (DHM) offers high resolution but often requires complex setups for large area imaging.

    Purpose of the Study:

    • To introduce a novel digital holographic microscopy technique for large area quantitative phase microscopy.
    • To develop a method that integrates specimen scanning and phase shift acquisition efficiently.
    • To demonstrate the versatility of the technique for various imaging applications.

    Main Methods:

    • Spatial phase scanning digital holography (SPSDH) involves scanning the object specimen across a tilted reference phase field.

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  • Camera frames are acquired at regular intervals during the single sweep.
  • A simple optomechanical system facilitates both the large area scan and phase shift acquisition simultaneously.

    • Main Results:

    • SPSDH enables large area quantitative phase imaging in a single continuous scan.
    • The technique achieves efficient phase shift acquisition through spatial scanning.
    • The optomechanical system is straightforward, simplifying the experimental setup.

    Conclusions:

    • Spatial phase scanning digital holography is a powerful new technique for large area QPM.
    • The method offers a practical solution for rapid, high-resolution phase imaging.
    • Potential applications include rapid analysis of blood smears, cell cultures, and microelectronic surfaces.