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Related Experiment Video

Updated: Dec 29, 2025

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
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Calcium Wave Promotes Cell Extrusion.

Yasuto Takeuchi1, Rika Narumi1, Ryutaro Akiyama2

  • 1Division of Molecular Oncology, Institute for Genetic Medicine, Hokkaido University Graduate School of Chemical Sciences and Engineering, 060-0815 Sapporo, Japan.

Current Biology : CB
|February 1, 2020
PubMed
Summary
This summary is machine-generated.

A calcium wave triggers the apical extrusion of damaged or transformed cells from epithelia, a process crucial for tissue health. This conserved mechanism involves specific channels and cell movements to maintain epithelial homeostasis.

Keywords:
INF2RasV12-transformedTRPC1actin rearrangementapoptosiscalcium wavecell extrusionepithelial homeostasis

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Tissue Homeostasis

Background:

  • Epithelial cells undergo apical extrusion when transformed or apoptotic to maintain tissue homeostasis.
  • The molecular mechanisms driving this essential cellular process remain largely unknown.

Purpose of the Study:

  • To elucidate the molecular mechanisms regulating apical extrusion of transformed and apoptotic cells from epithelia.
  • To identify key signaling pathways involved in cell extrusion.

Main Methods:

  • Utilized mammalian cultured epithelial cells and zebrafish embryos.
  • Investigated the role of calcium signaling, IP3 receptor, gap junctions, and TRPC1 in cell extrusion.
  • Analyzed cellular movements and actin rearrangements during extrusion.

Main Results:

  • Observed a calcium wave originating from transformed cells preceding their apical extrusion.
  • Demonstrated that this calcium wave triggers and facilitates cell extrusion.
  • Identified involvement of IP3 receptor, gap junction, and TRPC1 in calcium wave propagation.
  • Showed calcium waves induce polarized cell movement and actin rearrangement in surrounding cells.
  • Confirmed calcium wave's role in the apical extrusion of apoptotic cells.

Conclusions:

  • Calcium wave is a conserved, general regulatory mechanism for apical cell extrusion.
  • This pathway is critical for maintaining epithelial homeostasis by removing damaged or transformed cells.