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Mogroside V-producing Endophytic Fungi Isolated from Siraitia grosvenorii

Chen Bin1,2, Yu Fangming1,2, Jiang Zhi1,2

  • 1Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin, China.

Planta Medica
|February 1, 2020
PubMed

Insights

Endophytic fungi isolated from Siraitia grosvenorii produced mogroside V. Two fungal strains, Diaporthe angelica and Fusarium solani, were identified as key producers of this beneficial compound.

Area of Science:

  • Mycology
  • Plant Pathology
  • Natural Product Chemistry

Background:

  • Endophytic fungi, microorganisms residing within plant tissues, are recognized for their capacity to synthesize bioactive compounds.
  • Siraitia grosvenorii (monk fruit) is a valuable plant in traditional Chinese medicine and food, known for its sweet compounds.

Purpose of the Study:

  • To isolate and identify endophytic fungi from Siraitia grosvenorii capable of producing mogroside V.
  • To quantify the mogroside V yield from selected fungal isolates.

Main Methods:

  • Isolation and fermentation of 15 endophytic fungal strains from various parts of Siraitia grosvenorii.
  • Extraction of fermented broths using acetonitrile.
  • Analysis of extracts using High-Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) with mogroside V as a standard.
  • Identification of fungal isolates through morphological observation and Internal Transcribed Spacer (ITS) sequencing.

Main Results:

  • Two endophytic fungal strains, LHG-F5 and LHG-L4, were found to produce mogroside V.
  • Strain LHG-F5 yielded 3.814 µg/mL of mogroside V, while strain LHG-L4 yielded 2.675 µg/mL.
  • Isolate LHG-F5 was identified as Diaporthe angelica, and LHG-L4 as Fusarium solani.

Conclusions:

  • Endophytic fungi Diaporthe angelica and Fusarium solani, isolated from Siraitia grosvenorii, are capable of producing mogroside V.
  • These findings highlight the potential of endophytic fungi as a sustainable source for the production of valuable compounds like mogroside V.

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