One-day protocol for ¹⁸F-FDG and ¹³N-ammonia PET/CT with uptake decoupling score in differentiating untreated...

Accurate identification of low-grade gliomas (LGGs; World Health Organization grades I and II) and their differentiation from brain inflammation lesions (BILs) remains difficult; however, it is essential for treatment. This study assessed whether a one-day protocol for voxel-wise ¹⁸F-FDG and ¹³N-ammonia PET/CT with uptake decoupling analysis could differentiate LGGs from BILs.

Twenty-eight patients with LGGs and 16 patients with BILs underwent ¹⁸F-FDG and ¹³N-ammonia PET/CT on the same day before any type of therapy. The decoupling score and tumor-to-normal tissue (T/N) ratio of ¹⁸F-FDG and ¹³N-ammonia were calculated at each location. Student's t-test was used to compare values, and ROC curve analysis was used to establish a cut-off value for the T/N ratio and decoupling score. Area under the curve (AUC) was calculated to evaluate differential efficacy.

Significant differences were observed in ¹³N-ammonia T/N ratio (p=0.018) and decoupling score (p=0.003) between LGGs and BILs; however, the ¹⁸F-FDG T/N ratio did not show any differences (p=0.413). Optimal cut-off values for ¹⁸F-FDG T/N ratio, ¹³N-ammonia T/N ratio, and decoupling score were 0.73, 0.97, and 2.31, respectively, with corresponding AUCs of 0.48, 0.68, and 0.77. The respective sensitivity, specificity, and accuracy parameters using these cut-off values were 53.6%, 62.5%, and 56.8%, respectively, for ¹⁸F-FDG; 50.0%, 75.0%, and 59.1%, respectively, for ¹³N-ammonia; and 60.7%, 93.8%, and 72.7%, respectively, for decoupling score.

¹⁸F-FDG/¹³N-ammonia uptake decoupling score can be used to discriminate between LGGs and BILs. Use of a decoupling map of these two tracers can improve visual analysis and diagnostic accuracy.