Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

1.3K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
1.3K
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

2.8K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
2.8K
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

3.1K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
3.1K
Chromatographic Resolution01:15

Chromatographic Resolution

1.9K
In chromatography, a solute moves through a chromatographic column and tends to spread, forming a Gaussian-shaped band. The longer the solute spends in the column, the broader the band becomes. The broadening can lead to overlaps within the column, affecting separation effectiveness.
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...
1.9K
Diffusion on Chromatography Columns01:07

Diffusion on Chromatography Columns

1.2K
In column chromatography, when an analyte is introduced as a narrow band at the top of the column, the solutes begin to separate and broaden, developing a Gaussian profile. This broadening occurs due to various factors, such as longitudinal diffusion.
Longitudinal diffusion occurs when the solute molecules in the mobile phase diffuse from the more concentrated center of the chromatographic band to the more dilute regions on either side, both towards and against the flow direction. This...
1.2K
Column Efficiency: Rate Theory01:12

Column Efficiency: Rate Theory

826
The rate theory of chromatography provides quantitative insight into the shapes and widths of elution bands. These bands are based on the random-walk mechanism governing molecular migration within a column. The Gaussian profile of chromatographic bands arises from the cumulative effect of random molecular motions as they progress through the column.
During elution, a solute molecule experiences numerous transitions between stationary and mobile phases, exhibiting irregular residence times in...
826

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photonics-aided THz system for integrated secure communication and radar jamming.

Optics express·2026
Same author

Successful treatment of an ectopic pregnancy with elevated serum <i>β</i>-hCG levels (7,989.00 IU/L) using pure traditional Chinese medicine: a case report.

Frontiers in global women's health·2026
Same author

Photonics-Aided 20 m Wireless Transmission of 56-GBaud OFDM Signals at 138 GHz in the D-Band for 6G Applications.

Sensors (Basel, Switzerland)·2026
Same author

Regional downscaling increases projected changes of maritime continent large scale rainfall extremes.

Scientific reports·2026
Same author

RF pilot-aided frequency offset and phase noise compensation for photonics-aided D-band MIMO-OFDM systems.

Optics letters·2026
Same author

Experimental demonstration of a THz-to-photonics converter facilitated by electronic IQ mixers and a polarization-multiplexed intensity modulator.

Optics letters·2026
Same journal

Insights into cellulose/pectin effect in tobermorite/mandarin orange peel composite hydrogels and rehabilitation effect on Cd- and Pb-contaminated slopes.

Journal of chromatography. A·2026
Same journal

Retention prediction in reversed-phase liquid chromatography using XGBoost-based quantitative structure-retention relationships models.

Journal of chromatography. A·2026
Same journal

Impurity profiling of lipid-conjugated oligonucleotides using reversed-phase with and without ion-pair reagents and hydrophilic interaction liquid chromatography.

Journal of chromatography. A·2026
Same journal

Preparation of magnetic zwitterionic covalent organic frameworks for rapid simultaneous extraction of hydrophilic and hydrophobic organophosphates from environmental waters coupled with UHPLC-MS/MS determination.

Journal of chromatography. A·2026
Same journal

Analysis of organic and inorganic acids in biomass pyrolysis process samples by ion chromatography-mass spectrometry.

Journal of chromatography. A·2026
Same journal

Separation and enrichment of phages at the interface between two phases in a green solvent-based sugaring-out extraction system.

Journal of chromatography. A·2026
See all related articles

Related Experiment Video

Updated: Dec 29, 2025

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

36.4K

Peak compression in linear gradient elution liquid chromatography.

Weiqiang Hao1, Kai Wang2, Bangyi Yue3

  • 1School of Inspection and Testing Certification, Changzhou Vocational Institute of Engineering, Changzhou 213164, China; High-Tech Research Institute of Nanjing University, Changzhou 213164, China; Jiangsu Enzymatic Engineering Technology R&D Center, Changzhou 213164, China.

Journal of Chromatography. A
|February 2, 2020
PubMed
Summary
This summary is machine-generated.

A new method for calculating peak compression factor using temporal peak widths is introduced. This approach refines previous calculations by considering solvent effects, reducing discrepancies in peak width predictions.

Keywords:
Gradient elutionLinear solvent strengthLiquid chromatographyPeak compressionQuadratic solvent strength

More Related Videos

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
10:41

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization

Published on: April 5, 2019

18.6K
Using a Chemical Biopsy for Graft Quality Assessment
05:00

Using a Chemical Biopsy for Graft Quality Assessment

Published on: June 17, 2020

5.6K

Related Experiment Videos

Last Updated: Dec 29, 2025

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

36.4K
Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
10:41

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization

Published on: April 5, 2019

18.6K
Using a Chemical Biopsy for Graft Quality Assessment
05:00

Using a Chemical Biopsy for Graft Quality Assessment

Published on: June 17, 2020

5.6K

Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Peak compression factor is a critical parameter in chromatography.
  • Previous studies reported anomalous peak broadening, potentially due to procedural artifacts.

Purpose of the Study:

  • To propose a new expression for peak compression factor based on directly measurable temporal peak widths.
  • To reevaluate existing experimental data by incorporating solvent effects on retention and plate height.
  • To investigate the relationship between peak compression factors under ideal and real chromatographic conditions.

Main Methods:

  • Developed an expression for peak compression factor using temporal peak widths.
  • Reevaluated Neue's experimental data using the quadratic solvent strength model (QSSM).
  • Accounted for curvature in logarithmic retention factor (lnk) vs. mobile phase composition (φ) plots.
  • Incorporated variations in plate height (H) and isocratic peak width (lnWI) with φ using QSSM and quadratic equations.

Main Results:

  • The proposed method reduces the discrepancy between experimental and predicted peak widths.
  • Reevaluation confirms that anomalous peak broadening may be an artifact of theoretical peak width determination.
  • The relationship between ideal and real peak compression factors is discussed under linear gradient elution assumptions.

Conclusions:

  • The temporal peak width-based method offers a more accurate assessment of peak compression factor.
  • The study supports the hypothesis that previously observed peak broadening was likely procedural.
  • Understanding peak compression factors under various conditions is crucial for chromatographic method development.