Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

LP.8.1-directed COVID-19 mRNA vaccines durably boost neutralizing antibodies and mitigate ancestral immune imprinting.

PLoS pathogens·2026
Same author

LP.8.1-directed COVID-19 mRNA vaccines durably boost neutralizing antibodies and mitigate ancestral immune imprinting.

bioRxiv : the preprint server for biology·2026
Same author

Antibody evasion and receptor binding of SARS-CoV-2 LP.8.1.1, NB.1.8.1, XFG, and related subvariants.

Cell reports·2025
Same author

Antibody evasion and receptor binding of SARS-CoV-2 LP.8.1.1, NB.1.8.1, XFG, and related subvariants.

Cell reports·2025
Same author

WITHDRAWN: SA55 broadly neutralizes SARS-CoV-2 and robustly prevents viral escape by JN.1 sublineages.

bioRxiv : the preprint server for biology·2025
Same author

KP.2-based monovalent mRNA vaccines robustly boost antibody responses to SARS-CoV-2.

The Lancet. Infectious diseases·2025

Related Experiment Video

Updated: Dec 29, 2025

Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans
08:24

Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans

Published on: February 27, 2018

14.3K

Exploring Cryptococcus neoformans capsule structure and assembly with a hydroxylamine-armed fluorescent probe.

Conor J Crawford1, Radamés J B Cordero2, Lorenzo Guazzelli3

  • 1Centre for Synthesis and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland; Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, The Johns Hopkins University, Baltimore, Maryland 21205.

The Journal of Biological Chemistry
|February 2, 2020
PubMed
Summary

Researchers developed a fluorescent probe to study the Cryptococcus neoformans capsule. This chemical biology tool reveals insights into fungal virulence and capsule assembly at the cell surface.

Keywords:
aminooxy fluorescent probesbiosynthesiscapsulechemical biologyextracellular vesiclesfungal pathogenfungiglucuronoxylomannanglycobiologypolysaccharidereducing glycansvesiclesvirulence factor

More Related Videos

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body
08:08

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body

Published on: January 11, 2018

7.8K
Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions
10:56

Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions

Published on: June 22, 2019

6.7K

Related Experiment Videos

Last Updated: Dec 29, 2025

Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans
08:24

Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans

Published on: February 27, 2018

14.3K
Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body
08:08

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body

Published on: January 11, 2018

7.8K
Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions
10:56

Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions

Published on: June 22, 2019

6.7K

Area of Science:

  • Chemical biology
  • Mycology
  • Structural biology

Background:

  • Cryptococcus neoformans is an opportunistic fungal pathogen.
  • Its polysaccharide capsule is a key virulence factor but difficult to study.
  • Chemical biology offers tools to probe and understand biological systems.

Purpose of the Study:

  • To synthesize and apply a novel fluorescent probe for studying the C. neoformans capsule architecture.
  • To investigate the localization and accessibility of reducing glycans within the capsule.
  • To elucidate the role of reducing ends in capsule assembly and virulence.

Main Methods:

  • Synthesis of a hydroxylamine-armed fluorescent probe reactive with reducing glycans.
  • Application of the probe to C. neoformans under various conditions (e.g., capsule disruption).
  • Microscopic localization of probe signal and analysis of exopolysaccharides.

Main Results:

  • Probe signal localized intracellularly and at the cell wall-membrane interface, indicating reducing-end glycans at the capsule attachment site.
  • No signal detected in the capsule body, suggesting reducing ends are not exposed there.
  • Vesicle-like structures containing the probe were observed, supporting their role in capsule assembly.
  • Capsule disruption exposed reducing ends and compromised cell membrane integrity.
  • Isolated exopolysaccharides, unlike the assembled capsule, contained reducing ends.

Conclusions:

  • Reducing ends of glycans are localized at the cell wall surface, potentially serving as an initiation point for capsule assembly.
  • Chemical biology provides a powerful approach to study the C. neoformans capsule and its role in fungal virulence.
  • The findings propose a model for capsule assembly involving cell wall-associated reducing ends.