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Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma

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Published on: March 13, 2013

Targeting CSPG4 for isolation of melanoma cell-derived exosomes from body fluids

S Ferrone¹, T L Whiteside²

¹Department of Surgery, Massachusetts General Hospital, Harvard Medical School, 55 Fruit Street, 02114, Boston, MA, USA. sferrone@mgh.harvard.edu.

|February 2, 2020

Abstract:

This manuscript describes the functional properties of the exosomes released from melanoma cells. It details the characteristics of the tumor antigen chondroitin sulfate proteoglycan 4 (CSPG4), which is used as a marker to separate exosomes released by melanoma cells from exosomes released by nonmalignant cells. The results are discussed in view of the potential role of melanoma cell-derived exosomes in the escape of malignant cells from the host's immune system.

Keywords:

Antigens Extracellular vesicles Immune system Immunotherapy Neoplasm

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