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Updated: Dec 29, 2025

Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography

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Examining Immunotherapy Response Using Multiple Radiotracers.

Julian L Goggi¹, Siddesh V Hartimath², Youyi Hwang³

¹Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, #01-02 Helios, Singapore, 138667, Singapore. julian_goggi@sbic.a-star.edu.sg.

Molecular Imaging and Biology

|February 2, 2020

Summary

This summary is machine-generated.

A novel imaging agent, [68Ga]Ga-mNOTA-GZP, effectively predicts cancer immunotherapy response by correlating with CD8+ T cell changes. This biomarker aids in patient stratification and reduces treatment side effects.

Keywords:

Imaging Immune checkpoints Immunotherapy Positron emission tomography (PET)

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Positron Emission Tomography Imaging of Cell Trafficking: A Method of Cell Radiolabeling

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Published on: October 27, 2023

Area of Science:

Oncology
Immunology
Radiochemistry

Background:

Cancer immunotherapy offers significant potential but benefits only a subset of patients.
Non-invasive methods are crucial for stratifying responders due to immune-related side effects.
Current imaging lacks sensitivity for assessing immunotherapy efficacy and tumor microenvironment changes.

Purpose of the Study:

To evaluate radiopharmaceuticals as non-invasive biomarkers for predicting immunotherapy response.
To correlate radiotracer uptake with changes in the tumor microenvironment.
To assess biomarkers for stratifying response to PD-1 or combined PD-1/CTLA-4 therapy.

Main Methods:

Assessed [18F]FDG, [18F]FEPPA, [18F]FB-IL2, and [68Ga]Ga-mNOTA-GZP in a CT26-WT colon cancer model.
Correlated radiotracer tumor uptake with immune cell populations and response to immunotherapy.
Evaluated biomarkers for PD-1 monotherapy and combined anti-PD-1/CLTA-4 therapy.

Main Results:

[18F]FDG, [18F]FEPPA, and [18F]FB-IL2 showed limited ability to predict therapy response.
[68Ga]Ga-mNOTA-GZP demonstrated good predictive ability for immunotherapy response.
Uptake of [68Ga]Ga-mNOTA-GZP correlated well with changes in tumor-associated T cells, particularly CD8+ T cells.

Conclusions:

[68Ga]Ga-mNOTA-GZP shows promise as an imaging biomarker for stratifying immunotherapy response.
Grancyme B-based imaging agents warrant further development for patient stratification.
Early assessment with [68Ga]Ga-mNOTA-GZP can improve patient management and reduce side effects.