Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Dec 29, 2025

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation
10:15

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation

Published on: April 13, 2017

8.2K

Optimized siRNA Delivery into Primary Immune Cells Using Electroporation.

Mouldy Sioud1

  • 1Department of Cancer Immunology, Institute for Cancer Research, Oslo University Hospital-Radiumhospitalet, Oslo, Norway. mouldy.sioud@rr-research.no.

Methods in Molecular Biology (Clifton, N.J.)
|February 2, 2020
PubMed
Summary

This study shows that electroporation effectively delivers small interfering RNAs (siRNAs) into human monocytes and dendritic cells (DCs). This RNA delivery method enhances DC cancer vaccines, leading to strong anti-tumor immune responses.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering Immunity: Current Progress and Future Directions of CAR-T Cell Therapy.

International journal of molecular sciences·2026
Same author

RNA Therapeutics: Bridging Discovery and Clinical Implementation.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Molecular Mechanisms of Innate Immune Sensing of Exogenous RNAs.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Effects of Nucleoside Modifications on mRNA Translation: Choosing the Right Modifications.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Assessing the Immunogenicity of Synthetic RNA Using Blood Cells.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Large-Scale Production of Unmodified mRNA for Reprogramming Human Dendritic Cells and T Cells.

Methods in molecular biology (Clifton, N.J.)·2025

Area of Science:

  • Immunology
  • Molecular Biology
  • Biotechnology

Background:

  • Effective RNA delivery into immune cells is crucial for research and cancer immunotherapy.
  • Electroporation offers a safe and simple alternative to viral delivery for immune cell manipulation.

Purpose of the Study:

  • To evaluate nucleofection and electroporation for delivering small interfering RNAs (siRNAs) into primary human monocytes and dendritic cells (DCs).
  • To assess the efficacy of siRNA-loaded DC cancer vaccines in generating anti-tumor immune responses.

Main Methods:

  • Nucleofection and standard electroporation techniques were employed for siRNA delivery into human monocytes and DCs.
  • Development of DC cancer vaccines incorporating siRNA targeting immunosuppressive signals.
Keywords:
Cancer vaccinesDendritic cellsElectroporationImmunosuppressive factorsNucleofectionRNA interferencesiRNAs

More Related Videos

Preparation of Exosomes for siRNA Delivery to Cancer Cells
09:59

Preparation of Exosomes for siRNA Delivery to Cancer Cells

Published on: December 5, 2018

25.9K
High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA
09:14

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA

Published on: September 13, 2012

13.8K

Related Experiment Videos

Last Updated: Dec 29, 2025

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation
10:15

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation

Published on: April 13, 2017

8.2K
Preparation of Exosomes for siRNA Delivery to Cancer Cells
09:59

Preparation of Exosomes for siRNA Delivery to Cancer Cells

Published on: December 5, 2018

25.9K
High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA
09:14

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA

Published on: September 13, 2012

13.8K

Main Results:

  • Successful introduction of siRNAs into primary human monocytes and DCs was achieved using both nucleofection and electroporation.
  • DC cancer vaccines engineered with siRNA demonstrated the ability to induce robust and durable anti-tumor immunity.

Conclusions:

  • Electroporation and nucleofection are feasible methods for RNA delivery into human monocytes and DCs.
  • Targeted siRNA delivery via DC cancer vaccines shows promise for enhancing anti-tumor immune responses in cancer immunotherapy.