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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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What is Genetic Engineering?00:49

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Related Experiment Video

Updated: Dec 29, 2025

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells
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Engineering T Cells Using CRISPR/Cas9 for Cancer Therapy.

Xingying Zhang1,2,3, Chen Cheng1,3,4, Wen Sun1,3

  • 1State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Methods in Molecular Biology (Clifton, N.J.)
|February 2, 2020
PubMed
Summary

Gene editing technologies like CRISPR/Cas9 offer precise genome modification for T-cell cancer therapy. This review covers CRISPR/Cas9 applications, challenges, and future directions in enhancing T-cell therapies.

Keywords:
Adoptive cell therapyCRISPR-Cas9Gene editingImmunotherapyT cells

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Area of Science:

  • Biotechnology
  • Genomics
  • Immunology

Background:

  • Gene editing technologies, particularly CRISPR/Cas9, have advanced precise genomic modifications.
  • T-cell genetic engineering shows significant promise for improving cancer therapies.

Purpose of the Study:

  • To review the application of gene editing, specifically CRISPR/Cas9, in T-cell therapy.
  • To discuss the technical aspects, challenges, and future prospects of CRISPR/Cas9 in this field.

Main Methods:

  • Review of current literature on CRISPR/Cas9 applications in T-cell engineering.
  • Focus on technical capabilities including gene knockout, knock-in, and genome-wide screening.

Main Results:

  • CRISPR/Cas9 facilitates efficient gene knockout and site-specific knock-in in T cells.
  • The technology enables genome-wide screening for therapeutic target identification.

Conclusions:

  • CRISPR/Cas9 is a powerful tool for advancing T-cell-based cancer therapy.
  • Addressing current challenges will be crucial for realizing the full potential of these therapies.