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Lyophilized Autologous Serum Eyedrops: Experimental and Comparative Study.
José Santiago López-García1, Isabel García-Lozano2, Luis Rivas3
1Ophthalmology Service, Hospital Cruz Roja, Madrid, Spain.
American Journal of Ophthalmology
|February 2, 2020
Summary
Lyophilized autologous serum eyedrops maintain their biological stability and growth factor concentrations for at least one month. This process allows for easy storage and reconstitution for clinical use.
Area of Science:
- Ophthalmology
- Biotechnology
- Cell Biology
Background:
- Autologous serum eyedrops are used to treat ocular surface diseases.
- Lyophilization is a method of preserving biological materials.
- The stability of lyophilized autologous serum for ophthalmic use requires investigation.
Purpose of the Study:
- To analyze the biological stability of autologous serum eyedrops after lyophilization.
- To assess the impact of lyophilization and reconstitution on growth factor concentrations and biological activity.
- To evaluate the shelf-life of reconstituted lyophilized autologous serum.
Main Methods:
- Prospective, comparative experimental study involving serum from 12 healthy volunteers.
- Measurement of growth factors (TGF-β1, EGF, PDGF-AB) and albumin in fresh and lyophilized serum.
- Assessment of biological effects on conjunctival and corneal cell cultures, along with pH, osmolarity, and density measurements.
Main Results:
- No significant differences in growth factor concentrations between fresh and lyophilized serum.
- Growth factor concentrations remained stable for one month at 4°C after reconstitution.
- No changes in osmolarity, pH, or density; no adverse effects on cell cultures observed.
Conclusions:
- Lyophilization preserves the essential properties of autologous serum.
- Lyophilized autologous serum can be stored without strict temperature control and easily reconstituted.
- This method facilitates the standard clinical preparation and use of autologous serum eyedrops.
