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Using a Pan-Viral Microarray Assay Virochip to Screen Clinical Samples for Viral Pathogens

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Published on: April 27, 2011

Pathogenic viruses: Molecular detection and characterization.

I Made Artika¹, Ageng Wiyatno², Chairin Nisa Ma'roef²

¹Biosafety Level 3 Unit, Eijkman Institute for Molecular Biology, Jalan Diponegoro 69, Jakarta 10430, Indonesia; Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680, Indonesia.

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases

|February 2, 2020

Summary

Rapid detection and molecular characterization of pathogenic viruses are crucial for public health. Developing local laboratory capacity enhances the ability to identify and control emerging viral threats effectively.

Keywords:

DNA sequencing Pathogenic viruses Polymerase chain reaction Unidirectional workflow Virus detection

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Area of Science:

Virology
Molecular Biology
Public Health

Background:

Pathogenic viruses pose a significant and continuous threat to global public health due to their emergence and re-emergence.
Rapid detection and molecular characterization are essential for implementing control measures and limiting the spread of viral diseases.
Limited detection and characterization capacity in certain regions exacerbates the risk posed by potentially catastrophic viral outbreaks.

Purpose of the Study:

To review the molecular biology of pathogenic viruses.
To provide an overview of molecular techniques for virus detection and characterization.
To discuss principles of good laboratory practices for handling pathogenic viruses.

Main Methods:

Review of molecular biology principles relevant to pathogenic viruses.
Explanation of common molecular techniques such as PCR and DNA sequencing.
Discussion of essential good laboratory practices for safe virus handling.

Main Results:

Advances in molecular biology, particularly PCR and DNA sequencing, offer sensitive and specific methods for virus detection and circulation determination.
Understanding virus molecular biology is key to developing diagnostic tests and countermeasures.
Establishing local capacity for rapid virus identification is critical for global health security.

Conclusions:

Molecular biology techniques are vital tools for the detection and characterization of pathogenic viruses.
Adherence to good laboratory practices is paramount when working with pathogenic viruses.
Enhancing local laboratory capabilities is essential for effectively managing emerging viral threats worldwide.