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Sensitive and selective fluorescent probe for hypochlorite in 100% aqueous solution and its application for
Liang-Liang Gao1, Wan-Wan Wang1, Wei-Na Wu1
1College of Chemistry and Chemical Engineering, Henan Key Laboratory of Coal Green Conversion, Henan Polytechnic University, Jiaozuo 454000, PR China.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|February 3, 2020
Summary
A new pyrrole-cyanine probe detects hypochlorite (ClO-) with high selectivity and sensitivity. This fluorescent probe can be used for imaging hypochlorite in living cells.
Area of Science:
- Chemical sensing
- Fluorescent probes
- Analytical chemistry
Background:
- Hypochlorite (ClO-) is a reactive oxygen species involved in various biological processes.
- Developing selective and sensitive probes for ClO- detection is crucial for biological and medical research.
Purpose of the Study:
- To synthesize and characterize a novel morpholine-functionalized pyrrole-cyanine probe.
- To evaluate the probe's selectivity and sensitivity for ClO- detection.
- To investigate the mechanism of the probe's response to ClO-.
- To demonstrate the probe's utility in imaging ClO- in living cells.
Main Methods:
- Synthesis of a morpholine-functionalized pyrrole-cyanine probe via condensation reaction.
- Spectroscopic analysis (fluorescence and UV-vis) for probe characterization and ClO- detection.
- Electrospray ionization mass spectrometry (EIS-MS), Nuclear Magnetic Resonance (NMR), and Density Functional Theory (DFT) calculations for mechanism investigation.
- Live-cell imaging experiments to visualize ClO- in lysosomes.
Main Results:
- The probe was synthesized in high yield.
- The probe exhibited high selectivity for ClO- in aqueous solution, with fluorescence quenching and color change observed.
- A low detection limit of 0.165 μM for ClO- was achieved.
- The mechanism of hypochlorite-induced CC bond cleavage was elucidated.
- The probe successfully imaged ClO- within lysosomes of living cells.
Conclusions:
- The developed pyrrole-cyanine probe is a selective and sensitive tool for ClO- detection.
- The probe's mechanism involves CC bond breakage induced by hypochlorite.
- The probe is suitable for real-time imaging of ClO- in biological systems, particularly in lysosomes.
