Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Histone Variants at the Centromere02:30

Histone Variants at the Centromere

4.9K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.9K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

7.2K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
7.2K
Epigenetic Regulation01:37

Epigenetic Regulation

3.7K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.7K
Epigenetic Regulation01:46

Epigenetic Regulation

33.3K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.3K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.2K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.2K
Heterochromatin02:38

Heterochromatin

17.7K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
17.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual genomic localizations and gene regulatory functions of MBD-2 with and without NuRD in Caenorhabditis elegans which lacks DNA methylation.

Nature communications·2026
Same author

Aurora B/AIR-2 regulates sister centromere resolution and CENP-A/HCP-3 organization to prevent merotelic attachments.

Journal of molecular cell biology·2024
Same author

Argonaute protein CSR-1 restricts localization of holocentromere protein HCP-3, the C. elegans CENP-A homolog.

Journal of cell science·2024
Same author

Formation of artificial chromosomes in Caenorhabditis elegans and analyses of their segregation in mitosis, DNA sequence composition and holocentromere organization.

Nucleic acids research·2021
Same author

RbAp46/48LIN-53 and HAT-1 are required for initial CENP-AHCP-3 deposition and de novo holocentromere formation on artificial chromosomes in Caenorhabditis elegans embryos.

Nucleic acids research·2021
Same author

"Lessons from the extremes: Epigenetic and genetic regulation in point monocentromere and holocentromere establishment on artificial chromosomes".

Experimental cell research·2020

Related Experiment Video

Updated: Dec 29, 2025

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.5K

Epigenetic regulation of centromere function.

Charmaine Yan Yu Wong1, Bernard Chi Hang Lee1, Karen Wing Yee Yuen2

  • 1School of Biological Sciences, The University of Hong Kong, Kadoorie Biological Sciences Building, Pokfulam Road, Hong Kong, China.

Cellular and Molecular Life Sciences : CMLS
|February 3, 2020
PubMed
Summary
This summary is machine-generated.

Centromere function relies on epigenetic factors, not just DNA. This review explores how histone modifications and non-coding RNAs ensure accurate chromosome segregation through centromere protein A (CENP-A) assembly and propagation.

Keywords:
CentromereCentromeric transcriptHistone modificationsNon-coding transcriptionPericentric heterochromatinPost-translational modifications

More Related Videos

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A
09:02

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A

Published on: June 10, 2020

6.0K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.8K

Related Experiment Videos

Last Updated: Dec 29, 2025

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.5K
Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A
09:02

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A

Published on: June 10, 2020

6.0K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.8K

Area of Science:

  • Cell Biology
  • Epigenetics
  • Molecular Biology

Background:

  • Centromeres are crucial chromosomal regions for accurate segregation during cell division.
  • Centromere identity is epigenetically regulated, not solely determined by DNA sequence.
  • The histone H3 variant, centromere protein A (CENP-A), marks active centromeres.

Purpose of the Study:

  • To review the epigenetic mechanisms governing centromere formation and function.
  • To summarize the assembly and propagation of CENP-A during the cell cycle.
  • To discuss the interplay of post-translational modifications and non-coding RNAs in centromere maintenance.

Main Methods:

  • Literature review of studies on centromere biology and epigenetics.
  • Analysis of research on histone modifications and non-coding RNAs at centromeres.
  • Synthesis of current knowledge on CENP-A dynamics and function.

Main Results:

  • Centromere function is maintained by complex epigenetic regulation involving CENP-A.
  • Post-translational modifications of CENP-A and associated proteins are critical.
  • Histone modifications in centromeric and pericentric regions play key roles.
  • Non-coding transcription contributes to centromere integrity and function.

Conclusions:

  • Epigenetic factors, including histone modifications and non-coding RNAs, are essential for centromere function.
  • Understanding CENP-A dynamics and its regulatory network is key to comprehending chromosome segregation.
  • Further research into feedback mechanisms governing centromere epigenetics is warranted.