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Updated: Dec 29, 2025

Nitropeptide Profiling and Identification Illustrated by Angiotensin II
Published on: June 16, 2019
Human cationic amino acid transporters are not affected by direct nitros(yl)ation
Anastasia J Hobbach1, Ellen I Closs2
1Department of Pharmacology, University Medical Center of the Johannes Gutenberg University Mainz, Langenbeckstraße 1, 55131, Mainz, Germany.
Abstract:
A direct inhibiting effect of NO on the function of CAT-1 and -2A has been postulated to occur via nitrosylation of cysteine residues in the transporters. Neither the NO donor SNAP nor a mixture of SIN-1 and Spermine NONOate, that generates the strong nitrosating agent N2O3, reduced CAT-mediated L-arginine transport. Direct nitros(yl)ation does either not occur in CATs or does not affect their transport function. A regulatory effect of NO or nitrosating agents on CAT-mediated transport under physiological conditions seems, therefore, unlikely.
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