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Related Experiment Video

Updated: Dec 29, 2025

IR-TEx: An Open Source Data Integration Tool for Big Data Transcriptomics Designed for the Malaria Vector Anopheles gambiae
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IR-TEx: An Open Source Data Integration Tool for Big Data Transcriptomics Designed for the Malaria Vector Anopheles

Victoria A Ingham1, Andrew Bennett2, Duo Peng3

  • 1Vector Biology, Liverpool School of Tropical Medicine; Victoria.Ingham@lstmed.ac.uk.

Journal of Visualized Experiments : Jove
|February 4, 2020
PubMed
Summary

IR-TEx is a new tool for exploring gene expression linked to insecticide resistance in Anopheles gambiae mosquitoes. It aids in identifying new insecticide resistance genes, like GSTMS1, and understanding their functions.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Entomology

Background:

  • Insecticide resistance in mosquitoes is a major public health concern.
  • Understanding gene expression patterns is crucial for developing new control strategies.

Purpose of the Study:

  • To introduce IR-TEx, a Shiny application for exploring gene expression associated with insecticide resistance in Anopheles gambiae.
  • To provide a guide for modifying IR-TEx to incorporate new multi-omics datasets and handle missing data.
  • To demonstrate the utility of IR-TEx in identifying novel insecticide resistance candidate genes.

Main Methods:

  • Development of IR-TEx using the Shiny R package.
  • Exploration of transcript expression data linked to insecticide resistance phenotypes.
  • Modification of the local IR-TEx application to integrate new datasets and manage missing data.
  • Utilizing IR-TEx to identify candidate genes, exemplified by microsomal glutathione transferase (GSTMS1).

Main Results:

  • IR-TEx facilitates online and local exploration of gene expression related to insecticide resistance.
  • The application is adaptable for new multi-omics datasets and can be recoded for diverse experimental data.
  • IR-TEx successfully identified GSTMS1 as a transcript upregulated in pyrethroid-resistant Anopheles gambiae populations.
  • Co-correlated transcripts provided insights into the functional roles of GSTMS1.

Conclusions:

  • IR-TEx is a versatile and valuable resource for researchers studying insecticide resistance.
  • The tool aids in the discovery and functional characterization of insecticide resistance genes in mosquitoes.
  • GSTMS1 is highlighted as a potential key player in pyrethroid resistance in Anopheles gambiae.