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Related Concept Videos

Reproductive Cloning01:27

Reproductive Cloning

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Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...
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Related Experiment Video

Updated: Dec 29, 2025

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit
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Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning.

Pu Yan1, Yanjing Zeng1, Wentao Shen1

  • 1Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.

Frontiers in Bioengineering and Biotechnology
|February 4, 2020
PubMed
Summary

A new molecular cloning method, Nimble Cloning, uses SfiI and T5 exonuclease for efficient DNA assembly. This versatile technique simplifies gene expression and DNA construct modularization.

Keywords:
DNA assemblyGibson assembly-derived cloningmolecular cloningplant gene expressionprokaryotic expressionstandardized cloningvector construction

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Area of Science:

  • Molecular Biology
  • Biotechnology

Background:

  • Molecular cloning is a foundational technique in molecular biology.
  • Advances in biotechnology rely heavily on efficient molecular cloning methods.

Purpose of the Study:

  • To develop a novel, standardized method for molecular cloning.
  • To introduce the "Nimble Cloning" technique for versatile DNA assembly.

Main Methods:

  • Utilized the restriction enzyme SfiI and T5 exonuclease.
  • Simultaneously linearized vectors and generated 3'-overhangs.
  • Applied to both PCR products and plasmids for cloning.

Main Results:

  • Achieved highly efficient molecular cloning.
  • Demonstrated suitability for prokaryotic and eukaryotic gene expression.
  • Enabled reuse of DNA fragments and plasmid entry clones.
  • Applicable for single, multiple, and multi-site fragment cloning.

Conclusions:

  • Nimble Cloning offers a simple and versatile approach to molecular cloning.
  • The method has significant potential for modular DNA construct assembly.
  • Facilitates gene expression and DNA fragment manipulation.