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Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

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Published on: October 31, 2015

Picosecond-resolution phase-sensitive imaging of transparent objects in a single shot.

Taewoo Kim¹, Jinyang Liang¹, Liren Zhu¹

¹Caltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, California Institute of Technology, 1200 East California Boulevard, Mail Code 138-78, Pasadena, CA 91125, USA.

Science Advances

|February 4, 2020

Summary

Phase-sensitive compressed ultrafast photography (pCUP) enables single-shot, real-time imaging of transparent objects. This technique achieves high frame rates and sequence depths for observing ultrafast phenomena.

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Area of Science:

Optical Physics
High-Speed Imaging
Transparent Materials Science

Background:

High contrast and high frame rate imaging are crucial for studying ultrafast phenomena in transparent objects.
Existing phase-sensitive methods are limited by detector speeds and sequence depth.
Compressed Ultrafast Photography (CUP) offers high speed and sequence depth but lacks phase contrast.

Purpose of the Study:

To develop a novel imaging technique for single-shot, real-time observation of ultrafast phase dynamics in transparent media.
To overcome the limitations of current detectors in achieving high frame rates for phase-sensitive imaging.
To combine the benefits of dark-field imaging contrast with the speed and sequence depth of CUP.

Main Methods:

Development of phase-sensitive compressed ultrafast photography (pCUP).
Integration of dark-field imaging principles with the CUP technique.
Demonstration using optical Kerr effect and shock wave propagation experiments.

Main Results:

pCUP enables single-shot, real-time imaging of light-speed phase signals.
Achieved up to 350 frames captured at rates up to 1 trillion frames per second.
Successfully imaged complex ultrafast phenomena like shock wave propagation.

Conclusions:

pCUP is a powerful new tool for ultrafast phase-sensitive imaging of transparent objects.
The technique significantly advances the capabilities for studying transient optical events.
pCUP has broad applicability in fundamental and applied scientific research.