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Wnt Pathway Gene Expression Is Associated With Arterial Stiffness.

Allison L Kuipers1, Iva Miljkovic1, Emma Barinas-Mitchell1

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|February 5, 2020
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Summary

This study links Wnt pathway gene expression, specifically Adenomatous polyposis coli regulator of Wnt signaling pathway (APC) and transcription factor 4 (TCF4), to arterial stiffness in African ancestry men. These findings highlight a novel association in a population at high risk for vascular disease.

Keywords:
African ancestryWnt pathwayarterial stiffnessgene expression

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Area of Science:

  • Cardiovascular biology
  • Molecular genetics
  • Vascular health

Background:

  • The Wnt pathway is implicated in cardiovascular processes like fibrosis and atherosclerosis in animal models.
  • Human research on the Wnt pathway's role in cardiovascular health, particularly arterial stiffness, is limited.
  • African ancestry men are at increased risk for hypertensive vascular disease.

Purpose of the Study:

  • To investigate the association between peripheral blood Wnt pathway gene expression and arterial stiffness in healthy African ancestry men.
  • To identify specific Wnt pathway genes linked to arterial stiffness in this population.

Main Methods:

  • Gene expression of 43 Wnt pathway genes was measured in peripheral blood from 369 African ancestry men.
  • Arterial stiffness was assessed using brachial-ankle pulse-wave velocity.
  • Linear regression models were used to analyze associations between gene expression and pulse-wave velocity, adjusting for covariates.

Main Results:

  • Fourteen Wnt genes showed detectable expression.
  • Expression of Adenomatous polyposis coli regulator of Wnt signaling pathway (APC), glycogen synthase kinase 3β (GSK3B), and transcription factor 4 (TCF4) were significantly associated with arterial stiffness.
  • APC and TCF4 expression remained independently associated with arterial stiffness, each explaining approximately 3% of the variance.

Conclusions:

  • This study establishes a novel association between in vivo expression of Wnt pathway genes APC and TCF4 and arterial stiffness.
  • These findings suggest a potential molecular link between Wnt signaling and vascular health in African ancestry men.
  • Further research may explore therapeutic targets within the Wnt pathway for managing arterial stiffness.