Related Experiment Video
Updated: Dec 29, 2025
Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos
Published on: March 10, 2016
Establishment and Characterization of a Novel Primitive Yolk Sac Tumour Cell Line, TC587
Takeshi Iwasaki1,2, Kenichi Kohashi1, Masasuke Ohno3
1Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Background/Aim:
Yolk sac tumour (YST) is a rare malignant ovarian germ cell tumour that often occurs in young women or adolescents and exhibits an unfavourable outcome. To evaluate the biological behavior of carcinomas in vitro, permanent tumour cell lines are required. However, previously, only a few human YST cell lines have been established. Therefore, we aimed to establish a novel YST cell line.
Materials And Methods:
We established a novel YST cell line, TC587, from an adolescent patient with ovarian YST.
Results:
The cell line expressed AFP and SALL4, the characteristics of YST. In addition, we evaluated somatic mutations using next-generation sequencing and revealed some pathogenic variants, including mutations in the NRAS, KIT, KMT2C, RSF1, and TP53 genes.
Conclusion:
The newly established TC587 cell line may represent an effective tool for developing treatments and conducting molecular analyses for YST.
