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Production of Recombinant Adeno-Associated Viruses (rAAVs) by Transient Transfection
Cold Spring Harbor Protocols
|February 5, 2020
Abstract:
The most commonly used method for production of recombinant adeno-associated virus (rAAVs) in research laboratories is by transient triple transfection of 293 cells with AAV cis and trans plasmids and an adenovirus helper plasmid. This protocol describes the processes required to prepare the transfected cell suspension for virus purification.
