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Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
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Targeting proteins to the ER
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Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT
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Novel Tat-Dependent Protein Secretion.

Volkmar Braun1, Klaus Hantke2

  • 1Max Planck Institute for Developmental Biology, Department of Protein Evolution, Tübingen, Germany volkmar.braun@tuebingen.mpg.de.

Journal of Bacteriology
|February 5, 2020
PubMed
Summary
This summary is machine-generated.

Researchers discovered that the FecR protein, involved in ferric citrate transport, is secreted via the twin-arginine translocation (TAT) system. This finding reveals FecR as a novel type of bitopic membrane protein dependent on TAT for secretion.

Keywords:
iron regulationprotein secretiontransport

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Protein Secretion

Background:

  • Ferric citrate uptake in bacteria is regulated by a signaling pathway involving outer membrane protein FecA.
  • Signal transduction across the cytoplasmic membrane involves FecR protein and leads to the FecI extracytoplasmic function (ECF) sigma factor.
  • The mechanism of FecR protein secretion and its role in the signaling pathway were not fully understood.

Purpose of the Study:

  • To investigate the secretion pathway of the FecR protein.
  • To determine if FecR is secreted by the twin-arginine translocation (TAT) system.
  • To characterize FecR as a novel class of membrane protein.

Main Methods:

  • Sequence analysis of the FecR protein to identify signal motifs.
  • Experimental validation of FecR secretion using the TAT system.
  • Characterization of FecR as a bitopic membrane protein.

Main Results:

  • The FecR protein sequence contains the twin-arginine signal motif and a secretory (Sec) avoidance motif.
  • FecR is secreted by the TAT system, indicating a novel secretion pathway for this protein.
  • FecR is identified as a new class of bitopic Tat-dependent membrane proteins.

Conclusions:

  • The FecR protein utilizes the TAT system for secretion, a previously unrecognized pathway for this component of ferric citrate transport.
  • This study expands the known repertoire of proteins secreted by the TAT system and their functions.
  • The findings contribute to a deeper understanding of bacterial outer membrane protein transport and signal transduction mechanisms.