Related Experiment Videos
Liquid chromatographic assay for serum clonazepam
1Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison 53792.
Therapeutic Drug Monitoring
|January 1, 1988
Summary
This study presents an improved reversed-phase liquid chromatography assay for quantifying clonazepam in human serum. The method offers high accuracy and precision, minimizing interference for reliable anticonvulsant drug monitoring.
Area of Science:
- Analytical Chemistry
- Pharmacology
Background:
- Accurate quantification of anticonvulsant drugs like clonazepam is crucial for therapeutic drug monitoring.
- Existing analytical methods may suffer from interference and lack optimal efficiency.
Purpose of the Study:
- To develop and validate an improved reversed-phase liquid chromatographic assay for clonazepam in human serum.
- To enhance selectivity, speed, efficiency, and stability of the analytical method.
Main Methods:
- Serum samples were extracted using n-butyl chloride and back-extracted into HCl.
- Final extraction into chloroform/isopropanol followed by injection onto a cyanopropylsilane column.
- A specific mobile phase composition was used for optimal separation.
Main Results:
- The assay demonstrated linearity up to 400 µg/L with a detection limit of approximately 5 µg/L.
- High relative recovery (~100%) and excellent within-run and between-day precision (CV < 9%) were achieved.
- The method showed optimized selectivity, minimizing interference from other compounds and avoiding carbamazepine interference.
Conclusions:
- The developed reversed-phase liquid chromatographic assay provides a robust and reliable method for clonazepam quantification in human serum.
- This assay offers significant improvements in selectivity, speed, and efficiency compared to existing methods.