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Updated: Dec 29, 2025
miRNA Expression Analyses in Prostate Cancer Clinical Tissues
Published on: September 8, 2015
Identification of a potentially functional circRNA-miRNA-mRNA regulatory network for investigating pathogenesis and
Hong-Cheng Lu1, Jia-Qi Yao2, Xiao Yang1
11Department of Urology, The First Affiliated Hospital of Nanjing Medical University, 300, Guangzhou Road, Nanjing, 210029 Jiangsu China.
Background:
Circular RNAs (circRNAs) have received considerable attention in human cancer research. However, many circRNAs remain to be detected. In our study, we determined novel circRNAs and investigated their effects on bladder cancer (BCa).
Methods:
Microarray dataset GSE92675 was downloaded from Gene Expression Omnibus (GEO). Then, we combined computational biology with quantitative real-time polymerase chain reaction (qRT-PCR) to select related circRNAs in BCa. The selected circRNA-microRNA (miRNA)-messenger RNA (mRNA) regulatory subnetwork was determined by Gene Oncology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses.
Results:
The regulatory network constructed from the microarray dataset (GSE92675) contained 49 differentially expressed circRNAs (DECs). GO and KEGG analyses showed that the MAPK and PI3K-AKT signaling pathways were statistically significant. On the basis of qRT-PCR and the degree value calculated by the cytoHubba plugin of Cytoscape, hsa_circ_0011385 was finally confirmed. The subnetwork around hsa_circ_0011385 was constructed. In addition, we created a protein-protein interaction (PPI) network composed of 67 nodes and 274 edges after removing independent nodes. GO and KEGG analyses showed that hubgenes were involved in cell cycle activities. Moreover, they could be regulated by miRNAs and play an eventful role in BCa pathogenesis.
Conclusions:
We proposed a novel circRNA-miRNA-mRNA network related to BCa pathogenesis. This network might be a new molecular biomarker and could be used to develop potential treatment strategies for BCa.
Insights
This study identified novel circular RNAs (circRNAs) and their regulatory network involved in bladder cancer (BCa) pathogenesis. The findings suggest potential new biomarkers and therapeutic targets for BCa treatment.
Area of Science:
- Oncology
- Genomics
- Bioinformatics
Background:
- Circular RNAs (circRNAs) are increasingly recognized for their roles in human cancer.
- Many circRNAs in bladder cancer (BCa) remain undiscovered.
- This study aimed to identify novel circRNAs and explore their functions in BCa.
Purpose of the Study:
- To identify novel circRNAs associated with bladder cancer.
- To construct a circRNA-microRNA-messenger RNA (mRNA) regulatory network.
- To investigate the potential of these networks as biomarkers for BCa.
Main Methods:
- Utilized microarray dataset GSE92675 from Gene Expression Omnibus (GEO).
- Employed computational biology and quantitative real-time polymerase chain reaction (qRT-PCR) for circRNA selection.
- Performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses to determine regulatory subnetworks.
Main Results:
- Identified 49 differentially expressed circRNAs (DECs) and constructed a regulatory network.
- Significantly enriched pathways included MAPK and PI3K-AKT signaling.
- Confirmed hsa_circ_0011385 as a key molecule and built its associated subnetwork, revealing roles in cell cycle activities and BCa pathogenesis.
Conclusions:
- Proposed a novel circRNA-miRNA-mRNA network implicated in bladder cancer.
- This network represents a potential molecular biomarker for BCa.
- The findings may guide the development of novel therapeutic strategies for bladder cancer.
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