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Updated: Dec 29, 2025
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miRNA Expression Analyses in Prostate Cancer Clinical Tissues
Published on: September 8, 2015
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Gene expression dataset of prostate cells upon MIR205HG/LEADR modulation
Stefano Percio1, Federica Rotundo1, Paolo Gandellini1,2
1Department of Applied Research and Technological Development, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, 20133, Italy.
Data in Brief
|February 5, 2020
Summary
The host gene MIR205HG, now LEADR, acts independently of miR-205. This nuclear long noncoding RNA controls prostate basal cell differentiation, offering new research avenues.
Area of Science:
- Molecular Biology
- Genetics
- Cancer Research
Background:
- The function of MIR205HG, the host gene for miR-205, remains unclear.
- Investigated whether MIR205HG acts solely for miRNA production or has independent functions.
Purpose of the Study:
- To elucidate the independent role of MIR205HG in prostate basal cell differentiation.
- To reannotate MIR205HG as LEADR (Long Epithelial Alu-interacting Differentiation-related RNA).
Main Methods:
- Loss and gain of function experiments to modulate LEADR expression.
- Bioinformatic analysis of microarray data.
- Utilized multiple biologically independent replicates for silencing and overexpression strategies.
Main Results:
- Demonstrated LEADR's function as a nuclear long noncoding RNA.
- Showcased LEADR's ability to control basal-luminal differentiation in prostate cells.
- Validated data quality through high reproducibility and concordance with validation techniques.
Conclusions:
- LEADR plays a crucial miRNA-independent role in prostate differentiation.
- The dataset supports further research into MIR205HG/LEADR function and lncRNA mechanisms.
- Highlights the potential involvement of Alu sequences in lncRNA mechanisms.
