Imaging endogenous HClO in atherosclerosis using a novel fast-response fluorescence probe

Area of Science:

• Chemical Biology
• Biomedical Engineering
• Molecular Imaging

Background:

• Inflammatory diseases pose a significant health burden, necessitating advanced diagnostic tools.
• Current diagnostic methods can be invasive or lack sensitivity for early detection.
• Novel probes are needed for real-time monitoring and diagnosis of inflammation.

Purpose of the Study:

• To develop a novel pyrene-based probe for sensitive detection of inflammation.
• To evaluate the probe's utility in various biological systems, including cells, animal models, and human serum.
• To establish the probe as a potential tool for early diagnosis and monitoring of inflammatory diseases.

Main Methods:

• Synthesis of a novel pyrene-based naked-eye colorimetric and fluorescent turn-on probe (S-ClO).
• In vitro imaging studies using RAW264.7 cells.
• In vivo imaging studies using an arterial vessel inflammation nude mouse model.
• Application in human serum samples for disease monitoring.

Main Results:

• The developed probe (S-ClO) exhibited naked-eye colorimetric and fluorescent turn-on properties.
• Successful imaging of inflammation in living RAW264.7 cells.
• Effective visualization of arterial vessel inflammation in a nude mouse model.
• Demonstrated potential for analysis in human serum.

Conclusions:

• The S-ClO probe is a versatile tool for inflammation detection.
• Its dual-mode detection (colorimetric and fluorescent) enhances diagnostic capabilities.
• S-ClO shows significant promise for the early diagnosis and monitoring of inflammatory diseases.