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Updated: Dec 29, 2025
Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Published on: June 23, 2020
Detection of atherosclerosis-related hypochlorous acid produced in foam cells with a localized endoplasmic reticulum
Meng-Meng Zhang1, Yin-Hua Ma1, Peng Li2
1State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics (DICP), Chinese Academy of Sciences (CAS), Dalian 116023, P. R. China. klhan@dicp.ac.cn and University of the Chinese Academy of Sciences, Beijing 100049, P. R. China.
Abstract:
We have synthesized a turn-on fluorescent probe, termed NB4OH, to detect cellular hypochlorite. NB4OH is mainly localized in the endoplasmic reticulum and detects ClO- in foam cells. The fluorescence change of the probe was explained by theoretical calculation as a PET process. The probe holds great promise for application in biomedical research, including atherosclerosis research.
