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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

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MicroRNA‑27a‑3p promotes epithelial‑mesenchymal transition by targeting NOVA alternative splicing regulator 1 in

Kai Li1, Xiangrong Zhu2, Xihua Chen2

  • 1Department of Gastrointestinal Surgery II, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

Molecular Medicine Reports
|February 5, 2020
PubMed

Insights

NOVA1 acts as a tumor suppressor in gastric cancer. MicroRNA-27a-3p promotes cancer progression by inhibiting NOVA1, driving epithelial-mesenchymal transition (EMT) and leading to poor prognosis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Dysregulation of NOVA alternative splicing regulator 1 (NOVA1) is observed in gastric cancer.
  • Decreased NOVA1 expression correlates with gastric cancer progression and poor prognosis.
  • The role of NOVA1 in regulating epithelial-mesenchymal transition (EMT) in gastric cancer is not well understood.

Purpose of the Study:

  • To investigate the role of NOVA1 in gastric cancer.
  • To explore the relationship between NOVA1, miR-27a-3p, and EMT in gastric cancer.

Main Methods:

  • Analysis of miR-27a-3p expression in gastric cancer tissues and cell lines.
  • Overexpression and silencing experiments in AGS gastric cancer cells to assess effects on EMT and NOVA1 expression.
  • Immunohistochemical analysis of NOVA1 expression in 108 gastric cancer samples.

Main Results:

  • miR-27a-3p expression was elevated in gastric cancer and associated with shorter survival.
  • Overexpression of miR-27a-3p induced EMT and suppressed NOVA1 expression in AGS cells.
  • NOVA1 silencing promoted EMT in AGS cells.
  • Reduced NOVA1 expression in patient samples correlated with lymph node metastasis, advanced TNM stage, and poorer survival.

Conclusions:

  • NOVA1 functions as a tumor suppressor gene in gastric cancer.
  • miR-27a-3p promotes EMT by targeting and inhibiting NOVA1 in gastric cancer.
  • NOVA1 may serve as a prognostic biomarker for gastric cancer.
Keywords:
noVa alternative splicing regulator 1microrna-27a-3pgastric cancerepithelial-mesenchymal transition

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