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Updated: Dec 29, 2025
11:29
miRNA Expression Analyses in Prostate Cancer Clinical Tissues
Published on: September 8, 2015
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Long non-coding TUG1 accelerates prostate cancer progression through regulating miR-128-3p/YES1 axis
1Department of Urology, Zhejiang Integrated Traditional and Western Medicine Hospital/Hangzhou Red Cross Hospital, Hangzhou, China. banyingyanyaflo@163.com.
European Review for Medical and Pharmacological Sciences
|February 5, 2020
Summary
Taurine-upregulated gene 1 (TUG1) promotes prostate cancer (PCa) progression by interacting with miR-128-3p and YES1. Silencing TUG1 inhibits PCa cell growth and tumor development, suggesting TUG1 as a potential therapeutic target for PCa.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long non-coding RNAs (lncRNAs) are implicated in human cancers.
- Taurine-upregulated gene 1 (TUG1) shows potential oncogenic activity in prostate cancer (PCa).
Purpose of the Study:
- Investigate the molecular mechanisms of TUG1 in PCa progression.
- Determine the role of TUG1 in PCa cell proliferation, apoptosis, migration, and invasion.
Main Methods:
- Quantitative real-time PCR for gene expression analysis.
- MTT, flow cytometry, and Transwell assays for cell function assessment.
- Western blot, Dual-Luciferase reporter assays, and in vivo studies to elucidate molecular interactions and effects.
Main Results:
- TUG1 is upregulated in PCa and associated with poor prognosis.
- TUG1 knockdown inhibits PCa cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT), while promoting apoptosis.
- TUG1 interacts with miR-128-3p, which targets YES1, revealing a TUG1/miR-128-3p/YES1 regulatory axis.
Conclusions:
- TUG1 silencing retards PCa progression in vitro and in vivo via the miR-128-3p/YES1 pathway.
- Targeting TUG1 presents a novel therapeutic strategy for prostate cancer management.
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