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Updated: Dec 29, 2025
miRNA Expression Analyses in Prostate Cancer Clinical Tissues
Published on: September 8, 2015
MiRNA-215-5p alleviates the metastasis of prostate cancer by targeting PGK1
1Department of Urology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, China. chenjunyidoctor@163.com.
Objective:
MicroRNAs (miRNAs) are endogenous, non-coding RNAs, which exert crucial functions in regulating biological progressions. Previous studies have demonstrated the anti-tumor effect of miRNA-215-5p. However, its specific role in influencing the progression of prostate cancer (PCa) remains unclear. This study aims to uncover the regulatory effect of miRNA-215-5p on the metastasis and prognosis of PCa.
Patients And Methods:
MiRNA-215-5p levels in collected PCa tissues (n=52) and paracancerous tissues (n=52) were determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The relationship between miRNA-215-5p level and pathological indexes, as well as overall survival of PCa patients, was analyzed. Regulatory effects of miRNA-215-5p on proliferative and metastatic capacities of LNCaP and DU-145 cells were evaluated through cell counting kit-8 (CCK-8) and transwell assay, respectively. Bioinformatics prediction was performed to search for the target genes of miRNA-215-5p and PGK1 was selected. The biological role of PGK1 in the progression of PCa was finally clarified by a series of rescue experiments.
Results:
MiRNA-215-5p was lowly expressed in PCa tissues and cell lines. Low level of miRNA-215-5p predicted poor prognosis in PCa patients. The silence of miRNA-215-5p enhanced viability, migratory, and invasive capacities of LNCaP cells, while the overexpression of miRNA-215-5p yielded the opposite trends in DU-145 cells. PGK1 was predicted to be the target of miRNA-215-5p. PGK1 was upregulated in PCa tissues and cell lines and its high level predicted poor prognosis of PCa. Moreover, PGK1 level was negatively correlated to that of miRNA-215-5p in PCa tissues. PGK1 was able to reverse the regulatory effects of miRNA-215-5p on metastatic potentials of PCa cells.
Conclusions:
Downregulated miRNA-215-5p in PCa is closely related to distant metastasis and poor prognosis of affected patients. MiRNA-215-5p alleviates the malignant progression of PCa by targeting and downregulating PGK1.
Insights
Low levels of microRNA-215-5p (miRNA-215-5p) are linked to prostate cancer (PCa) metastasis and poor prognosis. MiRNA-215-5p inhibits PCa progression by targeting and downregulating phosphoglycerate kinase 1 (PGK1).
Area of Science:
- Molecular Biology
- Oncology
- Biochemistry
Background:
- MicroRNAs (miRNAs) are key regulators of biological processes.
- miRNA-215-5p has shown anti-tumor effects, but its role in prostate cancer (PCa) is not fully understood.
- Understanding miRNA-215-5p's function in PCa is crucial for developing new therapeutic strategies.
Purpose of the Study:
- To investigate the regulatory role of miRNA-215-5p in prostate cancer (PCa) metastasis.
- To determine the impact of miRNA-215-5p on the prognosis of PCa patients.
- To elucidate the molecular mechanism underlying miRNA-215-5p's function in PCa.
Main Methods:
- Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) to measure miRNA-215-5p and PGK1 expression in PCa tissues and cell lines.
- Cell counting kit-8 (CCK-8) and transwell assays to assess cell proliferation and metastasis.
- Bioinformatics analysis to predict miRNA-215-5p targets, followed by rescue experiments to validate PGK1's role.
Main Results:
- MiRNA-215-5p was significantly downregulated in PCa tissues and cell lines, correlating with poor prognosis.
- Downregulation of miRNA-215-5p promoted cell viability and metastasis, while its overexpression inhibited these processes.
- Phosphoglycerate kinase 1 (PGK1) was identified as a direct target of miRNA-215-5p, upregulated in PCa and associated with poor prognosis.
- PGK1 overexpression reversed the inhibitory effects of miRNA-215-5p on PCa cell metastasis.
Conclusions:
- Decreased miRNA-215-5p expression in PCa is associated with increased metastasis and unfavorable prognosis.
- MiRNA-215-5p suppresses prostate cancer progression by targeting and downregulating PGK1.
- Targeting the miRNA-215-5p/PGK1 axis may offer a novel therapeutic approach for prostate cancer.
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