Pseudolysobacter antarcticus gen. nov., sp. nov., isolated from soil in Fildes Peninsula, Antarctica

A novel strain, designated AQ6-296^T, was isolated from a soil sample collected in Fildes Peninsula, Antarctic. Cells were Gram-stain-negative, non-endospore-forming, non-motile, strictly aerobic and rod-shaped. Growth occurred at 4-28 °C (optimum, 20 °C) and at pH 6.0-7.0 (optimum, pH 7.0). NaCl was not obligatory for growth. Colonies were pale yellow after growth for 3 days at 20 °C on Reasoner's 2A agar. The strain was weakly positive for oxidase and the catalase test was negative. The only respiratory quinone was Q-8. The predominant cellular fatty acids were iso-C_16 : 0, iso-C_15 : 0, iso-C_11 : 0 3OH, summed feature 3 (comprising C_16 : 1ω7c and/or C_16 : 1ω6c) and summed feature 9 (comprising iso-C_17 : 1ω9c and/or C_16 : 010-methyl). The major polar lipids were phosphatidylethanolamine, unknown aminolipids, phosphatidylglycerol and diphosphatidylglycerol. The results of phylogenetic analysis based on 16S rRNA gene sequences (the highest similarity at 92.4 % to Lysobacter dokdonensis) indicated that strain AQ6-296^T is within the family Xanthomonadaceae. The DNA G+C content of the type strain was 58.6 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain AQ6-296^T is considered to represent a novel genus and species in the family Xanthomonadaceae, for which the name Pseudolysobacter antarcticus gen. nov., sp. nov. is proposed. The type strain is AQ6-296^T (CCTCC AB 2016313^T=KCTC 52744^T).