Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Particle Size-Driven Transition from Multilayer Aggregates to Ordered Monolayers at Gas Marble Interfaces.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Supraparticles with Enhanced Mechanical and Signal Stability for Identification of Objects.

ACS applied nano materials·2026
Same author

Quantifying Polydopamine Shell Hydration in Core-Shell Colloids by Analytical Ultracentrifugation: Implications for Self-Assembly and Structural Color of Photonic Crystals.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Emission enhancement of colloidal quantum dots confined in double disc nano-antennas with controlled opening.

Nanoscale·2025
Same author

Effect of Pore Structure of Inverse Opals on Wetting Transitions and Liquid Imbibition.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Self-Assembled Monolayers from Triethoxysilanes: Influence of Water Content and Chemical Structure on Formation Kinetics and Morphology.

Langmuir : the ACS journal of surfaces and colloids·2025

Related Experiment Video

Updated: Dec 29, 2025

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
09:35

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect

Published on: September 11, 2015

10.0K

Cell Interactions with Size-Controlled Colloidal Monolayers: Toward Improved Coatings in Bone Tissue Engineering.

Teresa Walter1, Alina Gruenewald2, Rainer Detsch2

  • 1Institute of Particle Technology , Friedrich-Alexander University Erlangen-Nürnberg , Cauerstrasse 4 , 91058 Erlangen , Germany.

Langmuir : the ACS Journal of Surfaces and Colloids
|February 5, 2020
PubMed
Summary

Surface feature size, not shape, critically impacts biomaterial interactions. Smaller features (<200 nm) enhance bone marrow stromal cell adhesion, spreading, and viability, improving implant material design.

More Related Videos

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
11:20

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Published on: March 29, 2018

8.0K
Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach
10:23

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

Published on: August 26, 2013

14.4K

Related Experiment Videos

Last Updated: Dec 29, 2025

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
09:35

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect

Published on: September 11, 2015

10.0K
Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
11:20

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Published on: March 29, 2018

8.0K
Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach
10:23

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

Published on: August 26, 2013

14.4K

Area of Science:

  • Biomaterials Science
  • Cellular Biology
  • Surface Engineering

Background:

  • Biomaterial surface structure is crucial for biological interactions.
  • Industrial processes often create particulate nanostructures on implants.
  • Understanding cellular responses to nanoscale topography is key for improved biomaterials.

Purpose of the Study:

  • To investigate the influence of controlled surface topography and feature size on cellular interactions.
  • To determine the role of feature size versus geometric details in cell adhesion and behavior.
  • To establish design criteria for particulate coatings on implantable biomaterials.

Main Methods:

  • Fabrication of surfaces with controlled topography (100-1000 nm) using colloidal templating.
  • Assessment of cell adhesion, spreading, viability, and activity using fluorescent microscopy and WST assay.
  • Morphology and kinetic analysis of bone-marrow derived murine stromal cells (ST2) on varied surfaces.

Main Results:

  • Cell adhesion, spreading, viability, and activity were significantly influenced by surface feature size.
  • Decreased feature size (<200 nm) enhanced cell adhesion and activity compared to flat surfaces.
  • Larger feature sizes (>200 nm) were detrimental to cell adhesion, with cells losing substrate contact over time.

Conclusions:

  • Colloidal templating provides a method for creating defined model systems to study cell functions.
  • Surface feature size is a critical design parameter for particulate coatings on biomaterials.
  • Optimizing feature size below 200 nm can improve the biological response of synthetic implant materials.