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Surface modified glass substrate for sensing E. coli using highly stable and luminescent CdSe/CdS core shell quantum

Chandan Hunsur Ravikumar1, Shwetharani R2, R Geetha Balakrishna2

  • 1Centre for Nano and Material Sciences, Jain Global Campus, Jain University, Bangalore 562112, India; Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkhuntien-Chaitalay Road, Thakam, Bangkok 10150, Thailand.

Journal of Photochemistry and Photobiology. B, Biology
|February 5, 2020
PubMed
Summary

Colloidal synthesis produced stable, water-soluble cadmium selenide/cadmium sulfide (CdSe/CdS) core-shell quantum dots (QDs). These biocompatible QDs detect E. coli bacteria with high sensitivity, showing potential for bio-imaging and sensing applications.

Keywords:
CdSe/CdSCore- shellE. coliGlass substrateQuantum yieldSynthesis

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Biotechnology

Background:

  • Quantum dots (QDs) offer unique optical properties for various applications.
  • Developing stable and biocompatible QDs is crucial for biological applications.
  • Efficient detection of pathogenic bacteria like E. coli remains a significant challenge.

Purpose of the Study:

  • To synthesize CdSe/CdS core-shell quantum dots (QDs) using a novel, low-temperature colloidal synthesis method.
  • To functionalize QDs for aqueous solubility and assess their biocompatibility.
  • To develop a sensitive QD-based biosensor for the detection of E. coli.

Main Methods:

  • Colloidal synthesis via hot injection technique with a binary ligand system and n-octadecane (nOD) solvent.
  • Characterization using X-ray diffraction (XRD) and transmission electron microscopy (TEM).
  • Modification with mercapto propionic acid (MPA) for aqueous solubility and cytotoxicity assessment (IC50).
  • Development of a sandwich assay using antibody-conjugated QDs and modified glass slides for E. coli detection.

Main Results:

  • Successful synthesis of CdSe/CdS core-shell QDs with red-shifted absorption and increased crystallite/particle size.
  • QDs exhibited high fluorescence intensity, stability, and good monodispersity.
  • Synthesized QDs demonstrated good biocompatibility with minimal toxicity (IC50 = 20 μg/L).
  • A QD-based biosensor achieved a limit of detection of 50 CFU/mL for E. coli.

Conclusions:

  • The developed low-temperature synthesis yields high-quality CdSe/CdS core-shell QDs.
  • Functionalized QDs are biocompatible and suitable for bio-imaging and sensing.
  • The QD-based sandwich assay provides a sensitive and effective method for E. coli detection.