Knockdown of P3H4 inhibits proliferation and invasion of bladder cancer

Area of Science:

• Oncology
• Molecular Biology
• Biochemistry

Background:

• Prolyl 3-hydroxylase family member 4 (P3H4), also known as SC65, is involved in various biological processes.
• The specific role of P3H4 in tumor development, particularly in bladder cancer (BC), remains largely unexplored.

Purpose of the Study:

• To investigate the function of P3H4 in bladder cancer progression.
• To elucidate the regulatory mechanisms controlling P3H4 expression in BC.

Main Methods:

• Analysis of P3H4 expression in BC tissues.
• In vitro and in vivo experiments involving P3H4 knockdown in BC cells.
• Chromatin immunoprecipitation (ChIP) assays to assess ETV4 binding to the P3H4 promoter.
• Overexpression studies of ETV4 in BC cells.

Main Results:

• P3H4 was found to be highly expressed in bladder cancer tissues.
• Silencing P3H4 significantly inhibited BC cell proliferation, cell cycle progression, migration, and invasion in vitro, and suppressed tumor growth in vivo.
• ETV4 directly binds to the promoter region of P3H4, activating its transcription.
• Overexpression of ETV4 counteracted the inhibitory effects of P3H4 knockdown on BC cell proliferation and invasion.
• ETV4 was significantly overexpressed in BC tissues.

Conclusions:

• P3H4 plays a crucial oncogenic role in the progression of bladder cancer.
• ETV4 directly regulates P3H4 transcription by binding to its promoter, contributing to bladder cancer development.