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Reduction of an in vitro Intraradicular Multispecies Biofilm Using Two Rotary Instrumentation Sequences.
Angelo Zavattini1, Jonathan Cowie2, Sadia Niazi1
1Department of Endodontics, Faculty of Dentistry, Oral & Craniofacial Sciences, Postgraduate Centre, Guy's Hospital, London Bridge, King's College London, London, United Kingdom.
European Journal of Dentistry
|February 5, 2020
Summary
Mechanical shaping effectively reduces intraradicular biofilm. Both the RaCe and ProTaper Gold systems demonstrated similar efficacy in biofilm reduction, highlighting the impact of mechanical cleaning in endodontics.
Area of Science:
- Endodontics
- Microbiology
- Dental Materials Science
Background:
- Intraradicular biofilm poses a significant challenge in endodontic treatment.
- Effective biofilm removal is crucial for successful root canal therapy.
- Novel shaping and cleaning techniques are continuously explored to improve biofilm eradication.
Purpose of the Study:
- To evaluate the efficacy of two shaping techniques, RaCe and ProTaper Gold, in reducing *in vitro* multispecies biofilm.
- To compare the biofilm reduction capabilities of RaCe and ProTaper Gold systems.
- To assess the impact of mechanical shaping alone on intraradicular biofilm.
Main Methods:
- Decoronated monoradicular teeth were used to create an *in vitro* biofilm model.
- Two instrumentation systems, RaCe and ProTaper Gold, were applied to root canal sections.
- Biofilm reduction was quantified by colony-forming unit counts and confocal laser scanning microscopy (CLSM).
Main Results:
- Both RaCe and ProTaper Gold instrumentation significantly reduced intraradicular biofilm.
- No statistically significant difference in biofilm reduction was observed between the RaCe and ProTaper Gold systems.
- Confocal laser scanning microscopy confirmed biofilm disruption and killing through mechanical shaping alone.
Conclusions:
- Mechanical shaping is effective in reducing intraradicular biofilm.
- The RaCe and ProTaper Gold systems exhibit comparable efficacy in biofilm reduction.
- Differences in file design and operational parameters did not lead to significant variations in biofilm removal between the tested systems.
