Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

8.3K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
8.3K
Genetic Screens02:46

Genetic Screens

5.5K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

New perspectives on lipid droplets in Arabidopsis leaves: biogenesis, resident proteins and functional diversity.

Journal of experimental botany·2026
Same author

Evolutionary perspectives on the development of the mammalian cerebellum: From cellular diversity to gene regulatory programs.

Current topics in developmental biology·2026
Same author

How growth mindset shapes math achievement: The moderating role of classroom practices and the mediating role of engagement across adolescence.

Developmental psychology·2026
Same author

CRISPR/Cas9 ribonucleoprotein-mediated knockout of <i>Gly m 4-L1</i> eliminates allergen accumulation in soybean.

Frontiers in plant science·2026
Same author

Actin fragmentation induced by Colletotrichum higginsianum infection facilitates hyphal invasion in Arabidopsis thaliana leaves.

Plant & cell physiology·2026
Same author

Preferential induction of Dt2 gene in the absence of E1 function enhances semi-determinate stem growth in soybean.

Journal of experimental botany·2026

Related Experiment Video

Updated: Dec 29, 2025

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
09:22

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

26.1K

A space-saving visual screening method, Glycine max FAST, for generating transgenic soybean.

Kosei Iwabuchi1,2, Takashi L Shimada3, Tetsuya Yamada4

  • 1Graduate School of Science, Kyoto University, Kyoto, Japan.

Plant Signaling & Behavior
|February 6, 2020
PubMed
Summary
This summary is machine-generated.

We developed Fluorescence-Accumulating Seed Technology (GmFAST) for faster soybean transformation. This method uses fluorescence to identify homozygous transgenic soybean seeds, saving space and time.

Keywords:
Arabidopsis thalianaGlycine maxGmFASToil bodyoleosinseed production

More Related Videos

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function
07:34

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

4.8K
Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
10:18

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

Published on: August 20, 2011

24.8K

Related Experiment Videos

Last Updated: Dec 29, 2025

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
09:22

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

26.1K
Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function
07:34

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

4.8K
Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
10:18

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

Published on: August 20, 2011

24.8K

Area of Science:

  • Plant Biotechnology
  • Genetics
  • Molecular Biology

Background:

  • Establishing homozygous transgenic soybean (Glycine max) lines is challenging and resource-intensive.
  • Conventional methods require significant time, space, and labor for screening and selection.

Purpose of the Study:

  • To develop an efficient, non-destructive method for selecting homozygous transgenic soybean plants.
  • To accelerate the development of improved soybean lines with desirable agronomic traits.

Main Methods:

  • Developed Fluorescence-Accumulating Seed Technology (GmFAST) using a seed-specific promoter and OLE1-GFP marker.
  • Utilized Agrobacterium-mediated transformation to introduce the marker into soybean cotyledonary nodes.
  • Selected homozygous transgenic seeds non-destructively using a fluorescence stereomicroscope.

Main Results:

  • GmFAST enabled identification of homozygous transgenic soybean seeds based on strong OLE1-GFP fluorescence.
  • The method reduced required growing space by 90% compared to traditional techniques.
  • Successfully generated a transgenic soybean line with increased seed pods and oil production, linked to Glyma13g30950 overexpression.

Conclusions:

  • GmFAST offers a space-saving, visual, and non-destructive screening approach for soybean transformation.
  • This technology enhances the efficiency of developing valuable soybean lines.
  • Identified a potential gene (Glyma13g30950) regulating soybean seed pod formation.