Classification of Andrographis paniculata extracts by solvent extraction using HPLC fingerprint and chemometric...

Area of Science:

• Phytochemistry
• Pharmacognosy
• Analytical Chemistry

Background:

• Andrographis paniculata is a traditional Indonesian antidiabetic remedy (jamu).
• Extraction solvent concentration impacts the yield of bioactive compounds.
• Standardized quality control is essential for consistent therapeutic effects.

Purpose of the Study:

• To develop a quality control method for Andrographis paniculata extracts.
• To evaluate the impact of different solvent extractions on bioactive compound content and antidiabetic activity.
• To utilize HPLC fingerprinting and chemometrics for extract analysis.

Main Methods:

• High-performance liquid chromatography (HPLC) fingerprint analysis.
• Chemometric analysis, specifically Principal Component Analysis (PCA).
• Quantification of andrographolide and α-glucosidase inhibition assay.

Main Results:

• HPLC fingerprints showed similar patterns across extracts, varying in peak intensity.
• PCA successfully classified Andrographis paniculata extracts based on solvent treatments.
• 50% ethanol extract yielded the highest andrographolide content and α-glucosidase inhibition; water extract yielded the lowest.

Conclusions:

• HPLC fingerprint analysis is effective for identifying Andrographis paniculata extracts based on extraction methods.
• This approach enhances quality control for therapeutic applications.
• Optimized extraction solvents can improve the antidiabetic efficacy of Andrographis paniculata.