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Silent hemoglobin variant during capillary electrophoresis: A case report

Yanping Yuan1, Xianghai Zhou1, Leili Gao1

  • 1Department of Endocrinology and Metabolism, Peking University People's Hospital, Beijing, China.

Journal of Diabetes Investigation
|February 6, 2020
PubMed

Hemoglobin (Hb) North Manchester [β51(D2) Pro→His; HBB:c.155 C>A] is a rare Hb β-globin gene variant that affects glycated Hb measurement values, such as ion-exchange high-performance liquid chromatography, in patients with diabetes. This variant was first detected in the UK in 1998. Here, we describe the first case involving Hb North Manchester detected incidentally in a patient with type 2 diabetes in Northern China. The Hb variant was discovered by ion-exchange high-performance liquid chromatography, yet capillary electrophoresis of both glycated Hb program and Hb program failed to detect it. Subsequently, Sanger sequencing was carried out to help identify the Hb variant.

Keywords:
Capillary electrophoresisGlycated hemoglobinHemoglobin variant

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