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Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
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Isoborneol Attenuates Low-Density Lipoprotein Accumulation and Foam Cell Formation in Macrophages

Yunfei Wang1,2, Zhengrong Li2, Boxue Liu1,2

  • 1State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai 810016, People's Republic of China.

Drug Design, Development and Therapy
|February 6, 2020
PubMed
Abstract

Insights

Isoborneol inhibits oxidized low-density lipoprotein (ox-LDL) uptake by macrophages, reducing lipid accumulation and preventing early atherosclerotic lesions. This finding reveals a novel mechanism for isoborneol in treating cardiovascular disease.

Area of Science:

  • Pharmacology
  • Biochemistry
  • Cardiovascular Research

Background:

  • Isoborneol is utilized in China for cardiovascular disease treatment, but its precise mechanism remains elusive.
  • Atherosclerosis, a cardiovascular disease, is characterized by lipid accumulation in macrophages, leading to lesion formation.

Purpose of the Study:

  • To elucidate the novel mechanism of isoborneol in the context of atherosclerotic disease.
  • To investigate isoborneol's effects on macrophage lipid uptake and accumulation.

Main Methods:

  • Whole-genome gene expression profiling of MCF-7 cells treated with isoborneol using mRNA microarray analysis.
  • Comparison of gene expression profiles with the Connectivity Map (CMAP) database.
  • Assessment of isoborneol's toxicity on RAW 264.7 cells via MTT assay, and evaluation of lipid accumulation and oxidized low-density lipoprotein (ox-LDL) uptake using Oil red O staining and Dil-ox-LDL assay.

Main Results:

  • Isoborneol exhibited mRNA expression profiles similar to ikarugamycin, a known inhibitor of ox-LDL uptake.
  • RAW 264.7 macrophages treated with isoborneol demonstrated reduced ox-LDL absorption and intracellular lipid accumulation.
  • These findings suggest isoborneol's potential to inhibit foam cell formation, a key event in early atherosclerosis.

Conclusions:

  • Isoborneol effectively inhibits macrophage uptake of ox-LDL, thereby preventing lipid accumulation.
  • This mechanism provides a scientific basis for the therapeutic application of isoborneol in managing atherosclerotic disease.
Keywords:
atherosclerosisconnectivity mapikarugamycinisoborneolmacrophageox-LDL

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