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Updated: Dec 29, 2025

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
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HPV-mediated down-regulation of NOD1 inhibits apoptosis in cervical cancer

Xubin Liu1, Hanyu Ma1, Lingyan Fei1

  • 1Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080 People's Republic of China.

Infectious Agents and Cancer
|February 6, 2020
PubMed

Insights

Nucleotide-binding oligomerization domain protein-1 (NOD1) is downregulated in cervical cancer, driven by HPV16 oncoproteins. NOD1 activation promotes apoptosis in HPV-positive cancer cells, suggesting its role in disease progression.

Area of Science:

  • Oncology
  • Immunology
  • Virology

Background:

  • Cervical cancer is a major global health concern, primarily caused by persistent high-risk Human Papillomavirus (hrHPV) infection.
  • Nucleotide-binding oligomerization domain protein-1 (NOD1) is an innate immune receptor involved in pathogen recognition and apoptosis, with known mutations in cancer cells.

Purpose of the Study:

  • To investigate the role of NOD1 in cervical cancer progression.
  • To determine the effect of HPV16 oncoproteins on NOD1 expression.
  • To evaluate the impact of NOD1 activation on HPV16-positive cervical cancer cell apoptosis.

Main Methods:

  • Analysis of NOD1 expression levels during cervical intraepithelial neoplasia and cervical cancer progression.
  • Investigation of HPV16 E6/E7 oncoprotein-induced NOD1 down-regulation.
  • Assessment of NOD1 activation effects on apoptosis in HPV16-positive cervical cancer cells.

Main Results:

  • NOD1 expression was found to decrease with the progression from cervical intraepithelial neoplasia to cervical cancer.
  • HPV16 E6/E7 oncoproteins were identified as inducers of NOD1 down-regulation.
  • Activation of NOD1 was shown to promote apoptosis in HPV16-positive cervical cancer cells.

Conclusions:

  • Dysregulation of NOD1-mediated inflammation and apoptosis may contribute to the progression of cervical intraepithelial neoplasia and cervical cancer.
  • NOD1 represents a potential therapeutic target for HPV-associated cervical malignancies.
Keywords:
ApoptosisCervical cancerCervical intraepithelial neoplasiaHPV16 E6/E7NOD1

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