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On comparing the reactivity of silver and lead, it is observed that the two ionic species, Ag+ (aq) and Pb2+ (aq), show a difference in their redox reactivity towards copper: the silver ion undergoes spontaneous reduction, while the lead ion does not. This relative redox activity can be easily quantified in electrochemical cells by a property called cell potential. This property is commonly known as cell voltage in electrochemistry, and it is a measure of the energy which accompanies the charge...
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Standardized protocol for determination of biohydrogen potential.

Julián Carrillo-Reyes1, Germán Buitrón1, Iván Moreno-Andrade1

  • 1Laboratory for Research on Advanced Processes for Water Treatment, Instituto de Ingeniería, Unidad Académica Juriquilla, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Queretaro, 76230, Mexico.

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|February 6, 2020
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Summary
This summary is machine-generated.

This study presents the first internationally validated protocol for assessing biohydrogen production potential (BHP). The standardized method ensures reliable and reproducible results across different laboratories, crucial for comparing experimental data.

Keywords:
Automatic protocolDark fermentationHeat-treated inoculumManual protocol

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Area of Science:

  • Biotechnology
  • Renewable Energy
  • Environmental Science

Background:

  • Biohydrogen production potential (BHP) assessment is vital for renewable energy research.
  • Current batch assay methods lack standardization, hindering data comparison.
  • Variability in protocols complicates evaluation of factors like inoculum and substrate.

Purpose of the Study:

  • To introduce the first internationally validated protocol for assessing biohydrogen production potential (BHP).
  • To establish quality criteria for BHP batch tests, ensuring reliable and reproducible results.
  • To provide two standardized protocol options (manual and automatic) for BHP assessment.

Main Methods:

  • International validation involving 8 independent laboratories across 5 countries.
  • Definition of quality criteria using coefficient of variation for cumulative hydrogen production (<15%).
  • Development and presentation of both manual (periodic measurement) and automatic (online measurement) BHP batch test protocols.

Main Results:

  • The validated protocol demonstrated acceptable repeatability and reproducibility.
  • Intra- and inter-laboratory coefficients of variation were within acceptable ranges.
  • The proposed method allows for a reduction in variability, with potential reductions up to 9%.

Conclusions:

  • The internationally validated protocol provides a standardized approach for BHP assessment.
  • The protocol enhances the reliability and comparability of biohydrogen research data.
  • Both manual and automatic options offer flexibility for different laboratory settings and measurement needs.