Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Dec 29, 2025

Decellularization and Recellularization Methodology for Human Saphenous Veins
11:35

Decellularization and Recellularization Methodology for Human Saphenous Veins

Published on: July 27, 2018

14.6K

Hyaluronic acid-heparin conjugated decellularized human great saphenous vein patches decrease neointimal thickness.

Hualong Bai1,2, Zhiwei Wang1, Mingxing Li1

  • 1Department of Vascular and Endovascular Surgery, First Affiliated Hospital of Zhengzhou University, Henan, China.

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
|February 6, 2020
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An international, multispecialty consensus document on the optimal management of patients with internal carotid artery occlusion.

Journal of vascular surgery·2026
Same author

Risk Factors for Delirium after Abdominal Aortic Aneurysm Repair.

Annals of vascular surgery·2026
Same author

Optimal treatment of preclinical cardiovascular disease: A narrative review with a focus on carotid artery stenosis.

Seminars in vascular surgery·2026
Same author

Skeletal Muscle Mass and Quality in Peripheral Artery Disease: New Insights into Clinical Management and Therapeutic Optimization.

Annals of vascular surgery·2026
Same author

From Acute Thrombosis to Eccentric Neointima in Arteriovenous Fistula.

Seminars in thrombosis and hemostasis·2026
Same author

Aortotomy-induced acute mural thrombosis progresses to saccular aneurysm formation.

Research and practice in thrombosis and haemostasis·2026
This summary is machine-generated.

Coating decellularized human great saphenous veins with hyaluronic acid-heparin creates a promising vascular graft. This tissue-engineered graft reduced blood clot formation and neointimal thickness in rat models, offering a durable solution for vascular surgery.

Area of Science:

  • Biomaterials Science
  • Vascular Surgery
  • Tissue Engineering

Background:

  • Graft failure remains a significant challenge in vascular surgery, necessitating durable solutions.
  • Coated prosthetic grafts and decellularization techniques offer potential advancements.
  • The feasibility of conjugating and implanting decellularized human saphenous vein is currently unknown.

Purpose of the Study:

  • To investigate the conjugation of hyaluronic acid-heparin to decellularized human great saphenous vein (GSV).
  • To evaluate the anti-thrombotic properties and efficacy of HA-heparin coated decellularized GSV patches in a rat model.
  • To assess the potential of tissue-engineered decellularized GSV as a vascular prosthesis.

Main Methods:

  • Human great saphenous veins were harvested, decellularized, and conjugated with hyaluronic acid-heparin.
Keywords:
angioplastygreat saphenous veinheparinmacrophagepatch

More Related Videos

Procedure for Human Saphenous Veins Ex Vivo Perfusion and External Reinforcement
09:50

Procedure for Human Saphenous Veins Ex Vivo Perfusion and External Reinforcement

Published on: October 1, 2014

11.7K
Patch Angioplasty in the Rat Aorta or Inferior Vena Cava
06:04

Patch Angioplasty in the Rat Aorta or Inferior Vena Cava

Published on: February 27, 2017

10.9K

Related Experiment Videos

Last Updated: Dec 29, 2025

Decellularization and Recellularization Methodology for Human Saphenous Veins
11:35

Decellularization and Recellularization Methodology for Human Saphenous Veins

Published on: July 27, 2018

14.6K
Procedure for Human Saphenous Veins Ex Vivo Perfusion and External Reinforcement
09:50

Procedure for Human Saphenous Veins Ex Vivo Perfusion and External Reinforcement

Published on: October 1, 2014

11.7K
Patch Angioplasty in the Rat Aorta or Inferior Vena Cava
06:04

Patch Angioplasty in the Rat Aorta or Inferior Vena Cava

Published on: February 27, 2017

10.9K
  • Characterization included water contact angle, morphology, and sulfur element analysis.
  • Patches were implanted into rat inferior vena cava and aorta, followed by analysis after 14 days.

    • Main Results:

    • Successful conjugation of HA-heparin to decellularized GSV was achieved, altering morphology and reducing water contact angle.
    • The HA-heparin coated GSV patch demonstrated in vitro anti-thrombotic properties.
    • Significant reduction in neointimal thickness was observed in both patch venoplasty and angioplasty models in rats.
    • CD90 and nestin positive cells were identified as participants in neointima formation.

    Conclusions:

    • HA-heparin coated decellularized human GSV patches effectively decrease neointimal thickness in both venous and arterial applications.
    • Tissue-engineered decellularized human GSV represents a promising vascular prosthesis with reduced thrombogenicity and improved tissue response.
    • This approach offers a potential durable solution to graft failure in vascular surgery.