Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Membrane protein structure and dynamics probed by MicroED.

Biochemical Society transactions·2026

Same author

pH-mediated activation of the lysosomal arginine sensor SLC38A9.

FEBS letters·2026

Same author

Direct from the seed: an atomic resolution protein structure by ab initio MicroED.

Nature communications·2026

Same author

Rapid Structural Analysis of Natural Products Using MicroED.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

β-barrels from short macrocyclic peptides.

Chemical communications (Cambridge, England)·2026

Same author

Conformational Trajectory of the Molecular Chameleon Grazoprevir From Formulation to Target-Bound.

Chemistry (Weinheim an der Bergstrasse, Germany)·2025

Same journal

Cryo-EM structure of the Arabidopsisthaliana ribosome in translating and non-translating states.

Structure (London, England : 1993)·2026

Same journal

Multifaceted effects of N-glycosylation on amyloidogenic κ light chains in AL amyloidosis.

Structure (London, England : 1993)·2026

Same journal

Near-complete cryo-EM structure of the Klebsiella pneumoniae podophage RAN69 reveals tail fiber-spike interface and a divergent pre-ejectosome.

Structure (London, England : 1993)·2026

Same journal

Saxiphilin is a broad-spectrum toxin sponge for C13-modified saxitoxins.

Structure (London, England : 1993)·2026

Same journal

Cryo-EM structure of YfdQ reveals a widespread family of bacteriophage-associated proteins with shell-like assemblies.

Structure (London, England : 1993)·2026

Same journal

An extracellular allosteric gate modulates the ligand efficacy of GPR119.

Structure (London, England : 1993)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 29, 2025

Laser Micro-Irradiation to Study DNA Recruitment During S Phase

Laser Micro-Irradiation to Study DNA Recruitment During S Phase

Published on: April 16, 2021

Experimental Phasing of MicroED Data Using Radiation Damage.

Michael W Martynowycz¹, Johan Hattne¹, Tamir Gonen¹

¹Howard Hughes Medical Institute, Departments of Biological Chemistry and Physiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA.

Structure (London, England : 1993)

|February 6, 2020

Summary

This summary is machine-generated.

This study demonstrates experimental phasing for atomic-resolution structures using microcrystal electron diffraction (MicroED) data alone. Radiation damage differences reveal heavy-atom sites, enabling peptide structure determination.

Keywords:

MicroED cryoEM heavy metal phasing radiation damage

More Related Videos

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments

Published on: June 27, 2022

Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells

Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells

Published on: September 5, 2017

Related Experiment Videos

Last Updated: Dec 29, 2025

Laser Micro-Irradiation to Study DNA Recruitment During S Phase

Laser Micro-Irradiation to Study DNA Recruitment During S Phase

Published on: April 16, 2021

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments

Published on: June 27, 2022

Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells

Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells

Published on: September 5, 2017

Area of Science:

Crystallography
Structural Biology
Electron Microscopy

Background:

Microcrystal electron diffraction (MicroED) enables atomic-resolution structure determination from small crystals.
Experimental phasing is crucial for solving unknown structures but challenging with MicroED data.

Purpose of the Study:

To demonstrate experimental phasing using solely MicroED data.
To solve the atomic-resolution structure of a peptide using radiation damage-induced differences.

Main Methods:

Continuous-rotation MicroED was performed twice on 11 crystals to collect low-dose and damaged datasets.
Differences in diffraction intensities between datasets were analyzed.
Patterson difference maps were used to identify heavy-atom sites and generate initial phases.

Main Results:

An experimentally phased structure of a seven-residue peptide was successfully determined.
The method utilized differences in diffraction intensities caused by radiation damage.
Iterative modeling and refinement completed the structure determination.

Conclusions:

MicroED data, combined with analysis of radiation damage effects, can be used for experimental phasing.
This approach facilitates the determination of atomic-resolution structures for small molecules and peptides.
The study validates MicroED as a powerful tool for structural biology.