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Related Experiment Video

Updated: Dec 29, 2025

Dissection and Culture of Mouse Embryonic Kidney

Dissection and Culture of Mouse Embryonic Kidney

Published on: May 17, 2017

Mouse Embryonic Stem Cell-Derived Ureteric Bud Progenitors Induce Nephrogenesis.

Zenglai Tan¹, Aleksandra Rak-Raszewska¹, Ilya Skovorodkin¹

¹Center for Cell Matrix Research, Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, Laboratory of Developmental Biology, Infotech Oulu, University of Oulu, Aapistie 5A, 90220 Oulu, Finland.

|February 7, 2020

Summary

Researchers developed an efficient method to generate ureteric bud progenitors from mouse embryonic stem cells. These cells successfully induced kidney organoid formation, aiding kidney development studies.

Keywords:

3D kidney organoids differentiation protocol mouse embryonic stem cell ureteric bud progenitor cells

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Last Updated: Dec 29, 2025

Dissection and Culture of Mouse Embryonic Kidney

Dissection and Culture of Mouse Embryonic Kidney

Published on: May 17, 2017

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney

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Published on: April 22, 2011

Author Spotlight: Optimizing iPSC Differentiation for Efficient Production to Generate Kidney Organoids

Author Spotlight: Optimizing iPSC Differentiation for Efficient Production to Generate Kidney Organoids

Published on: September 1, 2023

Area of Science:

Stem cell biology
Developmental biology
Regenerative medicine

Background:

Pluripotent stem cells (PSCs) offer a promising avenue for studying kidney development and disease.
Generating kidney organoids from PSCs is valuable for research.
Previous efforts in differentiating PSCs into ureteric bud (UB) progenitors have faced challenges.

Purpose of the Study:

To establish a simple, efficient, and reproducible protocol for differentiating mouse embryonic stem cells (mESCs) into UB progenitor cells.
To demonstrate the utility of mESC-derived UB cells in kidney organoid formation.

Main Methods:

Direct differentiation of mouse embryonic stem cells (mESCs).
Co-culture of mESC-derived UB progenitor cells with primary metanephric mesenchyme (pMM).

Main Results:

A straightforward and reproducible protocol successfully generated UB progenitor cells from mESCs.
The mESC-derived UB cells promoted nephrogenesis when co-cultured with pMM.
The resulting kidney organoids exhibited nephron structures and collecting ducts formed by the mESC-derived UB cells.

Conclusions:

This study provides an uncomplicated and reproducible platform for generating ureteric bud progenitors from mESCs.
The developed method facilitates the creation of kidney organoids for studying kidney development and disease.