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Updated: Dec 29, 2025

Harvesting of Peroneus Longus Tendon Autograft
Published on: September 2, 2025
The rule of twos: Technical note on consistent anatomical landmarks for hamstring graft harvesting
Lei Jiang1, Tay Kaesian1, Andrew Tan Hwee Chye1
1Department of Orthopaedic Surgery, Singapore General Hospital, Outram Road, 169608, Singapore.
Abstract:
We describe a novel hamstring harvesting technique using the "Rule of Twos" as an aide-memoire to dissect the conjoint tendon in an Asian population undergoing anterior cruciate ligament reconstruction. In a prospective study performed on 17 patients to validate this technique, a 2cm incision is placed 2cm distal and medial to the tip of the tibial tubercle. The surgeon then measured the following variables and calculated their means: 1) Distance from the tip of the tibial tuberosity to the proximal edge of the conjoint tendon (19.4 ± 1.4mm), 2) Width of the conjoint tendon insertion (18.8 ± 1.0mm), 3) Conjoint tendon length (20.1 ± 1.0mm).

