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Updated: Dec 29, 2025
The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events
Published on: January 7, 2019
Identification and evaluation of hub mRNAs and long non-coding RNAs in neutrophils during sepsis
Jiamin Huang1, Ran Sun1, Bingwei Sun2
1School of Medicine, Jiangsu University, Zhenjiang, 212001, Jiangsu Province, China.
Objective:
To reveal the systematic response of neutrophils to sepsis and to study the hub lncRNAs in sepsis.
Materials And Methods:
Neutrophils taken from the femur and tibia of male C57 BL/6 mice were used in this study. And neutrophils were treated for 0 h, 0.5 h, 1 h, and 4 h with or without 1 µg/mL lipopolysaccharide (LPS) for further chip detection. In addition, cecal ligation and perforation were used to simulate sepsis. Here, we used different bioinformatics analyses, including differential expression analysis, weighted gene co-expression network analysis (WGCNA), and gene regulatory network analysis, to analyze the systemic response of neutrophils to sepsis.
Results:
We identified nine modules and found hub lncRNAs in each module. The blue and pink modules were closely related to the inflammatory state of sepsis. Some hub lncRNAs (NONMMUT005259, KnowTID_00004196, and NR_003507) may have functions related to the inflammatory state in sepsis.
Conclusions:
Based on a new biological approach, our research results revealed the systemic-level response of neutrophils to sepsis and identified several hub lncRNAs with potential regulatory effects on this condition.
